Chlorite dismutases - a heme enzyme family for use in bioremediation and generation of molecular oxygen.
Chlorite dismutases - a heme enzyme family for use in bioremediation and generation of molecular oxygen.
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DOI：
10.1002/biot.201300210
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发表时间：
2014-04
影响因子：
4.7
通讯作者：
Obinger, Christian
中科院分区：
文献类型：
--
作者：
Hofbauer, Stefan;Schaffner, Irene;Furtmueller, Paul G.;Obinger, Christian
亚氯酸盐是一个严重的环境问题，因为在地下水、饮用水和土壤中检测到这种有害的人为化合物的浓度不断上升。因此，亚氯酸盐歧化酶（Clds）是生物修复中的重要分子，因为Clds催化亚氯酸盐降解为氯化物和分子氧。cld是存在于许多细菌和古细菌门中的含血红素b的氧化还原酶。本文综述了功能性Clds和Cld样蛋白的遗传学，并展示了这个新的酶家族与最近发现的染料脱色过氧化物酶的密切关系。可用的X-射线结构，生物物理和酶的性质，以及建议的反应机制，提出并严格讨论。解决有关结构-功能关系的公开问题，包括催化相关的氧化还原和反应中间体的性质和Clds在营业额的失活机制。在分析现有数据的基础上，建议将“Ditudatus Nitrospira defluvii”的Cld歧化酶作为未来生物技术和生物修复应用的模式Cld。此外，Clds可以在各种应用中用作分子氧的局部发生器，分子氧的反应性已经被必须在不存在分子氧的情况下进行有氧代谢途径的微生物利用。对于化学工程和生物修复领域的生物技术专家，本文综述了Cld酶家族的生物化学和生物物理背景，并对Cld的技术潜力进行了严格评估。
Chlorite is a serious environmental concern, as rising concentrations of this harmful anthropogenic compound have been detected in groundwater, drinking water, and soil. Chlorite dismutases (Clds) are therefore important molecules in bioremediation as Clds catalyze the degradation of chlorite to chloride and molecular oxygen. Clds are heme b-containing oxidoreductases present in numerous bacterial and archaeal phyla. This review presents the phylogeny of functional Clds and Cld-like proteins, and demonstrates the close relationship of this novel enzyme family to the recently discovered dye-decolorizing peroxidases. The available X-ray structures, biophysical and enzymatic properties, as well as a proposed reaction mechanism, are presented and critically discussed. Open questions about structure-function relationships are addressed, including the nature of the catalytically relevant redox and reaction intermediates and the mechanism of inactivation of Clds during turnover. Based on analysis of currently available data, chlorite dismutase from “Candidatus Nitrospira defluvii” is suggested as a model Cld for future application in biotechnology and bioremediation. Additionally, Clds can be used in various applications as local generators of molecular oxygen, a reactivity already exploited by microbes that must perform aerobic metabolic pathways in the absence of molecular oxygen. For biotechnologists in the field of chemical engineering and bioremediation, this review provides the biochemical and biophysical background of the Cld enzyme family as well as critically assesses Cld's technological potential.
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DOI：
10.1046/j.1432-1033.2002.03208.x
发表时间：
2002-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Hagedoorn, PL;de Geus, DC;Hagen, WR
通讯作者：
Hagen, WR
DOI：
10.1039/d3ta00655g
发表时间：
--
期刊：
Journal of Materials Chemistry A
影响因子：
9.5
作者：
Zhongheng Wang; Hui Xu; Zhiruo Liu; Mingzhe Jin; Linghao Deng; Sa Li; Yunhui Huang
通讯作者：
Zhongheng Wang
DOI：
10.1039/c2dt32312e
发表时间：
2013-03-07
期刊：
Dalton transactions (Cambridge, England : 2003)
影响因子：
--
作者：
Blanc B;Rodgers KR;Lukat-Rodgers GS;DuBois JL
通讯作者：
DuBois JL
影响因子：
2.9
作者：
Hofbauer S;Bellei M;Sündermann A;Pirker KF;Hagmüller A;Mlynek G;Kostan J;Daims H;Furtmüller PG;Djinović-Carugo K;Oostenbrink C;Battistuzzi G;Obinger C
通讯作者：
Obinger C
影响因子：
2.9
作者：
Ahmad, Mark;Roberts, Joseph N.;Bugg, Timothy D. H.
通讯作者：
Bugg, Timothy D. H.