FLOW VELOCITY IN THE SUPERFICIAL LYMPHATIC NETWORK OF THE MOUSE TAIL
FLOW VELOCITY IN THE SUPERFICIAL LYMPHATIC NETWORK OF THE MOUSE TAIL
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DOI：
10.1152/ajpheart.1994.267.4.h1507
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发表时间：
1994-10-01
影响因子：
4.8
通讯作者：
JAIN, RK
中科院分区：
文献类型：
--
作者：
LEU, AJ;BERK, DA;JAIN, RK
本研究有两个目的：1）建立一个动物模型，在该模型中可以在体内研究皮肤的初始毛细血管网络; 2）首次测量皮肤淋巴毛细血管网络中的有效流速（定义为流速的轴向分量）。使用荧光显微淋巴造影术技术对10只雌性动物（平均年龄45.8 ± 2.4天;平均体重21.2 ± 0.8 g）的裸鼠尾部皮肤浅层中的毛细淋巴管进行染色。利用密度图像分析，测量了沿尾部沿着的有效流速。该网络由一个六边形网格的蜂窝状层组成，可以在所有动物中染色。有效淋巴液流速范围为1.4-20.4 μ m/s，平均值为7.7 +/- 5.9 μ m/s;中位数为6.2 μ m/s（4.5-10.5; 25和75%流速）。这种新的动物模型允许研究皮肤中的淋巴毛细血管的大网络，并且应该提供对初始炎症的生理学和病理生理学的新见解。
The present study had two goals: 1) to establish an animal model in which a large network of the initial lymphatics of the skin can be investigated in vivo and 2) to measure effective flow velocity (defined as axial component of the flow velocity) in the lymph capillary network of the skin for the first time. A fluorescence microlymphography technique was used to stain the lymph capillaries in the superficial layer of the skin of the nude mouse tail in 10 female animals (mean age 45.8 +/- 2.4 days; mean wt 21.2 +/- 0.8 g). With the use of densitometric image analysis, effective flow velocity along the tail was measured. The network consisted of a honeycomb-like layer of hexagonally shaped meshes that could be stained in all animals. Effective lymph flow velocities were in the range of 1.4-20.4 mu m/s with a mean value of 7.7 +/- 5.9 mu m/s; median value was 6.2 mu m/s (4.5-10.5; 25 and 75% percentiles). This new animal model allows studies of a large network of lymph capillaries in the skin and should provide new insight into the physiology and pathophysiology of the initial lymphatics.