Comment 2 on Staessen et al. (2004). Two-cell biopsy and PGD pregnancy outcome.
Comment 2 on Staessen et al. (2004). Two-cell biopsy and PGD pregnancy outcome.
复制标题
对 Staessen 等人的评论 2
DOI：
10.1093/humrep/deh880
复制
发表时间：
2005
影响因子：
6.1
通讯作者：
S. Munné
中科院分区：
文献类型：
--
作者：
Jacques Cohen;S. Munné
非整倍体的植入前遗传学诊断（PGD）的效率和可靠性与卵子或胚胎活检的阶段、应用程序的技巧、相对于发育阶段移除的细胞数量以及评估的染色体数量密切相关。不仅实际的PGD程序和选定的患者群体在PGD测试的最终成功中发挥作用，而且活检的影响可能会对结果产生不利影响，这取决于特定试管婴儿实验室操作的一般情况。换句话说，在某些情况下，卵裂胚胎的活组织检查可能会对存活产生负面影响，即使只有一个细胞被移除。它取决于操作条件——不同实验室的操作条件差别很大——以及胚胎培养条件——差别更大！但当多个细胞被移除时，它肯定会受到不成比例的影响。认为PGD成功的报告往往缺乏对患者和实验室差异的更好理解，在描述整体情况时也不够坦率；他们没有考虑到病人护理、技术应用和临床相关差异之间的复杂平衡。正是在这种背景下，我们想对Staessen等人（2004）的研究发表评论。本研究通过活组织检查描述PGD的作用
The efficiency and reliability of preimplantation genetic diagnosis (PGD) for aneuploidy is closely linked to the stage at which eggs or embryos are biopsied, the finesse with which the procedures are applied, the number of cells removed with respect to developmental stage, and the number of chromosomes assessed. Not only do the actual PGD procedure and the selected patient population play roles in the final success of the PGD test, but the impact of biopsy may adversely affect outcome dependent on the general circumstances in which a given IVF laboratory operates. In other words, biopsy of the cleaved embryo may in some instances alter the survival negatively even if only one cell is removed. It is dependent on conditions of manipulation—which vary widely from laboratory to laboratory—and embryo culture—which varies even more!—but it certainly is affected disproportionately when more than one cell is removed. Reports arguing the success of PGD often lack a greater comprehension of patient and laboratory differences and candour when describing the whole picture; they fail to consider the intricate balance between patient care, application of technology and clinic-related differences. It is in this context that we would like to comment on the study of Staessen et al.(2004). This study describes the effect of PGD by biopsying