BIOCHEMICAL EFFECTS OF ANTIBODIES AGAINST NERVE GROWTH-FACTOR ON DEVELOPING AND DIFFERENTIATED SYMPATHETIC-GANGLIA
BIOCHEMICAL EFFECTS OF ANTIBODIES AGAINST NERVE GROWTH-FACTOR ON DEVELOPING AND DIFFERENTIATED SYMPATHETIC-GANGLIA
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DOI：
10.1016/0006-8993(78)90401-8
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
THOENEN, H
中科院分区：
文献类型：
--
作者：
GOEDERT, M;OTTEN, U;THOENEN, H
神经生长因子（NGF）是交感神经元正常发育和维持其功能所需的蛋白质。阐明其生理作用的主要方法是分析外源性施用NGF的作用和表征通过施用单特异性抗NGF抗体中和内源性NGF的结果。新生动物的抗血清导致周围交感神经系统14的几乎完全和不可逆的破坏，关于抗NGF抗体对完全分化的交感神经元的作用知之甚少2，本研究旨在观察单剂量纯化的抗神经生长因子抗体对上级颈神经节（SCG）内儿茶酚胺合成酶的影响在不同发育阶段的老鼠。此外，我们通过测量胆碱乙酰转移酶（CAT）的水平，研究了节后神经元中抗NGF抗体影响的变化如何通过节前胆碱能纤维的改变来反映，所述胆碱乙酰转移酶（CAT）是一种已知在节前胆碱能神经末梢9中定位于95 °以上的酶，并且是胆碱能神经元的可靠标记。
Nerve growth factor (NGF) is a protein which is required for the normal development and maintenance of function of sympathetic neuronslL A major approach to the elucidation of its physiological role represents the analysis of the effects of exogenously administered NGF and the characterization of the consequences of the neutralization of endogenous NGF by administration of monospecific anti NGF-antibodies.Although it is well known that the administration of NGF-antiserum to newborn animals leads to an almost complete and irreversible destruction of the peripheral sympathetic nervous system 14, much less is known about the effects of anti NGF-antibodies on fully differentiated sympathetic neurons2, L It was the aim of the present study to investigate the effects of a single dose of purified anti NGF-antibodies on the catecholamine-synthesizing enzymes in the superior cervical ganglion (SCG) of the rat at different stages of the development. Moreover, we investigated how the changes effected by anti NGF-antibodies in the postganglionic neurons are reflected by alterations in the preganglionic cholinergic fibers by measuring the level of choline acetyltransferase (CAT), an enzyme which is known to be localized to more than 95~ in the preganglionic cholinergic nerve terminals 9 and which represents a reliable marker for cholinergic neurons.