Prevalence of toxocariasis in northeastern Brazil based on serology using recombinant Toxocara canis antigen
Prevalence of toxocariasis in northeastern Brazil based on serology using recombinant Toxocara canis antigen
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DOI：
10.4269/ajtmh.2005.72.103
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发表时间：
2005-01-01
影响因子：
3.3
通讯作者：
Yamasaki, H
中科院分区：
文献类型：
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作者：
Coelho, RDL;De Carvalho, LB;Yamasaki, H
为了评估巴西东北部伯南布哥省Jaboatao dos Guararapes地区儿童弓形虫病的患病率，采用重组犬弓形虫抗原，采用酶联免疫吸附试验（ELISA）检测了215份血清样本。在215例受试者中，有26例（12.1%）呈阳性。在使用215个血清样本中的53个进行点印迹分析时，诊断结果与ELISA获得的结果相关。此外，ELISA法证实重组犬弓形虫抗原对弓形虫病具有高度特异性。考虑到重组抗原对弓形虫病的特异性，在各种寄生虫感染普遍流行的热带和亚热带地区，推荐使用重组犬弓形虫抗原进行ELISA或点印迹检测。
To evaluate the prevalence of toxocariasis in children in Jaboatao dos Guararapes, Pernambuco in northeastern Brazil, 215 serum samples were examined by an enzyme-linked immunosorbent assay (ELISA) using a recombinant Toxocara canis antigen. In the ELISA, 26 (12.1%) of 215 subjects were positive. In a dot-blot assay using 53 of 215 serum samples, the diagnostic results correlated with those obtained by the ELISA. Moreover, it has been confirmed that the recombinant T. canis antigen was highly specific for toxocariasis by ELISA using serum samples positive for antibody to Ascaris limbridicoides. Considering the specificity of the recombinant antigen to toxocariasis, the ELISA or dot-blot assay using the recombinant T. canis antigen is recommended in tropical and sub-tropical regions where various parasitic infections are commonly endemic.