CD4+CD25high regulatory T cells in human autoimmune diabetes
CD4+CD25high regulatory T cells in human autoimmune diabetes
复制标题
DOI：
10.1016/j.jaut.2004.11.004
复制
发表时间：
2005-02-01
影响因子：
12.8
通讯作者：
Gottlieb, PA
中科院分区：
文献类型：
--
作者：
Putnam, AL;Vendrame, F;Gottlieb, PA
在小鼠模型中，CD4(+)CD25(+)T细胞参与维持外周免疫耐受。在人类中，最近描述了一种表达高水平CD25的CD4+CD25+T细胞亚群(CD4(+)CD25(高))，其特征与小鼠CD4(+)CD25(+)相同。我们检测了1型糖尿病患者(T1D)和正常对照组(NC)外周血中CD4(+)CD25(High)T细胞的特征。与以前的报告相比，我们发现T1D和NC的CD4(+)CD25(高)和CD4(+)CD25(+)T细胞的数量没有差异。我们证实了以前的研究表明，无论有没有抗CD28抗体，人CD4(+)CD25(High)细胞都能抑制在亚极交联剂抗CD3刺激下共培养的CD4(+)CD25(-)细胞的增殖。然而，我们没有观察到正常对照组和我们测试的慢性糖尿病受试者之间的统计差异。这些细胞群体的培养似乎没有影响它们在两组中抑制增殖的能力。总之，我们发现在慢性人类T1D受试者中，CD4(+)CD25(HIGH)的数量或体外调节功能没有显著差异。(C)2004爱思唯尔有限公司。保留所有权利。
In mouse models, CD4(+)CD25(+) T cells are involved in maintenance of peripheral tolerance. In humans, a subset of CD4+CD25+ T cells expressing high levels of CD25 (CD4(+)CD25(high)) with characteristics identical to murine CD4(+)CD25(+) was recently described. We evaluated the characteristics of CD4(+)CD25(high) T cells in peripheral blood of type 1 diabetic subjects (T1D) and normal controls (NC). In contrast to a previous report, we found no difference in the number of CD4(+)CD25(high) and CD4(+)CD25(+) T cells between T1D and NC. We confirmed previous studies that demonstrated that human CD4(+)CD25(high) cells can suppress the proliferation of co-cultured CD4(+)CD25(-) cells stimulated in conditions of sub-maximal cross-linking by anti-CD3 either with or without anti-CD28. However, we did not observe statistical differences between the normal controls and the chronic diabetic subjects we tested. Culturing of these cell populations did not appear to affect their ability to suppress proliferation in both groups. In conclusion, we found no significant differences in number or in vitro regulatory function of CD4(+)CD25(high) in chronic human T1D subjects. (c) 2004 Elsevier Ltd. All rights reserved.