Detection of non-polio enteroviruses in Hungary 2000-2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30
Detection of non-polio enteroviruses in Hungary 2000-2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30
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DOI：
10.1007/s11262-009-0440-4
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发表时间：
2010-04-01
期刊：
影响因子：
1.6
通讯作者：
Berencsi, Gyoergy
中科院分区：
文献类型：
--
作者：
Kapusinszky, Beatrix;Szomor, Katalin N.;Berencsi, Gyoergy
人类肠道病毒与各种临床综合征相关，从轻微的发热性疾病到严重的、可能致命的疾病，如无菌性脑膜炎、瘫痪、心肌炎和新生儿肠道病毒败血症。 2000年6月至2008年8月期间，匈牙利报告了埃可病毒(E)2型、4型、6型、7型、9型、11型、13型、25型、30型、柯萨奇病毒(CV)-A16、-A19、-B5和肠道病毒71型(EV71)。在这项研究中，对来自 28 名被诊断患有手足口病、脑膜炎和脑炎的患者的 29 份既往肠道病毒阳性样本进行了分子分型。通过对编码衣壳蛋白 VP1 的基因组区域直接测序来评估已鉴定血清型 CV-A16、EV71 和 E30 的遗传关系。将这些序列相互比较，并与 Genbank 中拥有的其他地理区域的序列进行比较。 CV-A16 的系统发育分析表明，这些病毒大多起源于远东或亚太地区。 EV71的分型显示，2000年的一种病毒属于C1基因型，2004年和2005年观察到的5种病毒被鉴定为C4基因型。 11 种埃可病毒 30 株与欧洲邻国的病毒株表现出同源性。 E30 的分子检查显示，2000 年、2001 年和 2004-2006 年在匈牙利传播了三个不同的谱系。
Human enteroviruses are associated with various clinical syndromes from minor febrile illness to severe, potentially fatal conditions like aseptic meningitis, paralysis, myocarditis, and neonatal enteroviral sepsis. Between June 2000 and August 2008 echovirus (E) type 2, 4, 6, 7, 9, 11, 13, 25, 30, coxsackievirus (CV) -A16, -A19, -B5, and enterovirus 71 (EV71) were reported in Hungary. In this study, 29 previously enterovirus positive samples from 28 patients diagnosed with hand, foot and mouth disease, meningitis and encephalitis, were molecularly typed. The genetic relationships of identified serotypes CV-A16, EV71, and E30 were assessed by direct sequencing of genomic region encoding the capsid protein VP1. The sequences were compared to each other and sequences from other geographical regions possessed in Genbank. The phylogenetic analysis of CV-A16 revealed that the viruses were mostly of Far-Eastern or Asia-Pacific origin. Typing of EV71 showed that one virus from 2000 belonged to genotype C1 and five viruses observed in 2004 and 2005 were identified as genotype C4. The 11 echovirus 30 strains showed homology with those of neighbor European countries. The molecular examination of E30 revealed that three separate lineages circulated in 2000, 2001, and 2004-2006 in Hungary.