Study of the thermal stability of glucose oxidase in the presence of water-soluble polymers
Study of the thermal stability of glucose oxidase in the presence of water-soluble polymers
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DOI：
10.1002/app.12845
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发表时间：
2003-10-31
影响因子：
3
通讯作者：
Letskovska, Z
中科院分区：
文献类型：
--
作者：
Godjevargova, T;Vasileva, N;Letskovska, Z
在28~60℃温度范围内，研究了葡萄糖氧化酶(GOD)在含水解聚丙烯腈(HPAN)和聚氧乙烯(POE)溶液中的热稳定性。将计算结果与GOD在无聚合物溶液中的热稳定性进行了比较。研究发现，所研究的聚合物提高了酶的热稳定性。研究了水溶性聚合物浓度对酶热稳定性的影响。3wt%的HPAN溶液和1wt%的聚氧乙烯溶液对酶的热稳定性保护效果最好。浓度较高的溶液会导致该酶迅速失活。结果表明，3wt%的HPAN溶液比1wt%的POE溶液效果更好(59.0比52.0%)。用差示扫描量热法(DSC)研究了HPAN存在和不存在时酶的热转变。在HPAN存在下，上帝的熔化温度升高了11摄氏度。这支持了这样的假设，即HPAN增加了上帝的热稳定性。(C)2003年威利期刊公司。
The thermal stability of glucose oxidase (GOD) in solutions containing water-soluble hydrolyzed polyacrylonitrile (HPAN) and polyoxyethylene (POE) was studied as a function of time and temperature between 28 and 60degreesC. The results were compared with the thermal stability of GOD in solutions without polymers. The polymers studied were found to increase the enzyme thermal stability. The influence of the concentration of the water-soluble polymers on enzyme thermal stability was also studied. The best protection effect on enzyme thermal stability had 3 wt % solution of HPAN and 1 wt % solution of polyoxyethylene. Solutions with higher concentrations led to a quick deactivation of the enzyme. It was proved that the effect of 3 wt % HPAN solution was stronger than the effect of 1 wt % POE (59.0 versus 52.0%). The thermal transition of the enzyme was studied in both the presence and the absence of HPAN by DSC. The melting temperature of GOD in the presence of HPAN was shifted to an 11degreesC higher value. This sustained the supposition that HPAN increases the thermostability of GOD. (C) 2003 Wiley Periodicals, Inc.