Secondary cross infection with dengue virus serotype 2/3 aggravates vascular leakage in BALB/c mice
Secondary cross infection with dengue virus serotype 2/3 aggravates vascular leakage in BALB/c mice
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登革热病毒血清型 2/3 继发交叉感染加重 BALB/c 小鼠血管渗漏
DOI：
10.1002/jmv.27826
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发表时间：
2022-05-14
影响因子：
12.7
通讯作者：
Sun，Qiangming
中科院分区：
文献类型：
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作者：
Qiu，Lijuan;Huang，Xinwei;Sun，Qiangming
中国说，登革热病毒(DV)在云南偶尔会出现流行水平。疫苗的开发受到抗体依赖增强和缺乏良好动物模型的限制。因此，本研究调查了BALB/c小鼠基于母体免疫的交叉感染，并评估了DV2-D13113和DV3-YNWS2流行病毒株交叉感染的风险。DV在BALB/c幼鼠的器官内复制，甚至导致死亡。特别是，如果母亲感染了DV2，感染DV3的幼鼠患严重疾病的风险更高。虽然BALB/c成虫和幼鼠在感染DV2/DV3后存活5-8天并产生抗DV抗体，但继发DV感染后可见广泛的皮下血管渗漏。此外，在异型病毒感染母亲所生的后代中，肺和肾脏的血管渗透性显著增加。因此，血管渗漏表明有严重的DV感染。结果表明，母体免疫增加了随后异型感染的严重程度。此外，D13113和YNWS2的继发性交叉感染代表着严重疾病的风险。这项研究对DV交叉感染的研究和疫苗的开发具有重要意义。
Dengue virus (DV) has occasionally emerged at epidemic levels in Yunnan, China. Vaccine development is limited by antibody‐dependent enhancement and a lack of good animal models. Thus, the study investigated cross infection based on maternal immunity in BALB/c mice and assessed the risk of cross infection by DV2‐D13113 and DV3‐YNWS2 epidemic virus strains. DV replicated within the organs of the BALB/c infant mice, even causing death. Particularly, DV3‐infected infant mice were at higher risk of severe disease if their mothers were infected with DV2. Although BALB/c adults and pups survived DV2/DV3 infection and produced anti‐DV antibodies after 5–8 days, extensive subcutaneous vascular leakage was observed after secondary DV infection. Furthermore, vascular permeability in the lung and kidney significantly increased in offspring born to heterotypic virus‐infected mothers. Thus, vascular leakage indicates severe DV infection. The results indicate that maternal immunity increases the severity of subsequent heterotypic infection. Additionally, secondary cross infection by D13113 and YNWS2 represents a risk of serious disease. This study has implications for studies of DV cross infection and vaccine development.