Stimulus encoding by specific inactivation of cortical neurons.
Stimulus encoding by specific inactivation of cortical neurons.
复制标题
通过皮质神经元的特异性失活编码刺激。
DOI：
10.1038/s41467-024-47515-x
复制
发表时间：
2024
影响因子：
16.6
通讯作者：
Yuste,Rafael
中科院分区：
文献类型：
--
作者：
Pérez-Ortega,Jesús;Akrouh,Alejandro;Yuste,Rafael
Neuronal ensembles are groups of neurons with correlated activity associated with sensory, motor, and behavioral functions. To explore how ensembles encode information, we investigated responses of visual cortical neurons in awake mice using volumetric two-photon calcium imaging during visual stimulation. We identified neuronal ensembles employing an unsupervised model-free algorithm and, besides neurons activated by the visual stimulus (termed “onsemble”), we also find neurons that are specifically inactivated (termed “offsemble”). Offsemble neurons showed faster calcium decay during stimuli, suggesting selective inhibition. In response to visual stimuli, each ensemble (onsemble+offsemble) exhibited small trial-to-trial variability, high orientation selectivity, and superior predictive accuracy for visual stimulus orientation, surpassing the sum of individual neuron activity. Thus, the combined selective activation and inactivation of cortical neurons enhances visual encoding as an emergent and distributed neural code.
