Two Putative Polysaccharide Deacetylases Are Required for Osmotic Stability and Cell Shape Maintenance in Bacillus anthracis.
Two Putative Polysaccharide Deacetylases Are Required for Osmotic Stability and Cell Shape Maintenance in Bacillus anthracis.
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DOI：
10.1074/jbc.m115.640029
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发表时间：
2015-05-22
期刊：
影响因子：
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通讯作者：
Bouriotis V
中科院分区：
文献类型：
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作者：
Arnaouteli S;Giastas P;Andreou A;Tzanodaskalaki M;Aldridge C;Tzartos SJ;Vollmer W;Eliopoulos E;Bouriotis V
背景：BA0330和BA0331是炭疽芽孢杆菌11种已知或推测的多糖脱乙酰酶中仅有的两种脂蛋白。结果：两种蛋白均缺乏脱乙酰酶活性，对细菌的细胞形态维持(BA0331)或高盐胁迫适应(BA0330)具有重要作用。结论：BA0330和BA0331具有稳定炭疽杆菌细胞壁的作用。意义：了解脂蛋白维持细胞壁完整性的机制。膜锚定的脂蛋白具有广泛的功能，并在革兰氏阳性菌的几个细胞过程中发挥关键作用。BA0330和BA0331是炭疽芽孢杆菌11种已知或推测的多糖脱乙酰酶中仅有的两种脂蛋白。我们发现这两种脂蛋白都表现出独特的特征。BA0330和BA0331与肽聚糖相互作用，BA0330对于细菌在高浓度盐存在下的生长是重要的，而BA0331有助于维持均匀的细胞形状。它们似乎不会改变肽聚糖的结构，也不会对溶菌酶产生抗性。BA0330的高分辨X射线结构显示了一个C-末端结构域和一个该家族所特有的N-末端结构域，该结构域由一个结构类似于纤维连接蛋白3型结构域的两层(4+3)β-夹心结构组成。我们的数据表明，BA0330和BA0331在稳定炭疽杆菌细胞壁方面具有结构性作用。
Background: BA0330 and BA0331 are the only two lipoproteins among 11 known or putative polysaccharide deacetylases from Bacillus anthracis. Results: Both proteins lack deacetylase activity and are important for cell shape maintenance (BA0331) or high salt stress adaptation of the bacterium (BA0330). Conclusion: BA0330 and BA0331 stabilize the cell wall of B. anthracis. Significance: Understanding the mechanisms by which lipoproteins maintain cell wall integrity. Membrane-anchored lipoproteins have a broad range of functions and play key roles in several cellular processes in Gram-positive bacteria. BA0330 and BA0331 are the only lipoproteins among the 11 known or putative polysaccharide deacetylases of Bacillus anthracis. We found that both lipoproteins exhibit unique characteristics. BA0330 and BA0331 interact with peptidoglycan, and BA0330 is important for the adaptation of the bacterium to grow in the presence of a high concentration of salt, whereas BA0331 contributes to the maintenance of a uniform cell shape. They appear not to alter the peptidoglycan structure and do not contribute to lysozyme resistance. The high resolution x-ray structure of BA0330 revealed a C-terminal domain with the typical fold of a carbohydrate esterase 4 and an N-terminal domain unique for this family, composed of a two-layered (4 + 3) β-sandwich with structural similarity to fibronectin type 3 domains. Our data suggest that BA0330 and BA0331 have a structural role in stabilizing the cell wall of B. anthracis.