QUANTITATIVE-ANALYSES OF CONSTITUENT MEMBRANES OF PAROTID ACINAR CELLS AND OF CHANGES EVIDENT AFTER INDUCED EXOCYTOSIS
QUANTITATIVE-ANALYSES OF CONSTITUENT MEMBRANES OF PAROTID ACINAR CELLS AND OF CHANGES EVIDENT AFTER INDUCED EXOCYTOSIS
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DOI：
10.1007/bf00307161
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
WILLIAMS, MA
中科院分区：
文献类型：
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作者：
COPE, GH;WILLIAMS, MA
在异丙肾上腺素(IPR)诱导的兔腮腺分泌的EM水平上进行了形态计量学研究。这里的重点是腺泡细胞的膜内容物和诱导脱颗粒后发生的变化。特别是，人们希望从数量形态的角度确定酶原颗粒膜作为完整的电子显微镜可见亚单位保存并随后重新利用是否是一个可信的假说。两小时后，IPR已导致颗粒的95%耗尽。在此期间，约有1343μm~2/cell的颗粒限制膜与顶端质膜暂时融合，2小时后，1158μ~2/cell的颗粒限制膜已被清除。我们没有发现酶原颗粒膜以光滑膜碎片的形式无限期地储存在高尔基体区域或细胞质的其他地方。IPR引起粗面内质网膜面积的变化(+37.7%，1406cell~2/μ)是可能的，但我们认为颗粒膜的位置不可信。
A morphometric study has been made at the EM level of Isoproterenol (IPR) induced secretion of rabbit parotid glandsin vivo. Emphasis has been placed here on the membrane content of acinar cells and the changes which occur following induced degranulation. In particular it was hoped to establish whether the preservation of zymogen granule membrane as intact electron microscopically visible subunits and the subsequent reutilisation of this membrane is a plausible hypothesis from a quantitative morphological standpoint.After two hours IPR had caused >95% depletion of granules. About 1343 μm2/cell of granule limiting membrane temporarily fused with the apical plasmalemma during this time and by two hours 1158 μm2/cell of this had been eliminated. Only a small increase in intracellular smooth membrane area was recorded after degranulation and we find no evidence that the zymogen granule membrane is stored indefinitely as smooth membrane fragments either in the region of the Golgi apparatus or elsewhere in the cytoplasm.IPR caused changes in RER membrane area (+37.7%, 1406 μm2/cell), which is a possible, but we consider implausible relocation site of granule membrane.The possible mechanism of the removal of ‘excess apical membrane’ and the ultimate fate of the zymogen granule membrane is discussed.