Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.
Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.
复制标题
脂肪酸合酶 2 通过调节脂质积累和胁迫耐受基因表达，有助于甲虫的滞育准备
DOI：
10.1038/srep40509
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发表时间：
2017-01-10
影响因子：
4.6
通讯作者：
Wang XP
中科院分区：
文献类型：
--
作者：
Tan QQ;Liu W;Zhu F;Lei CL;Wang XP
滞育，也被称为休眠，是一种发育受阻的状态，允许昆虫在不利的环境条件下生存。滞育昆虫在为滞育做准备时会储存大量脂肪。然而，这些累积的脂肪储备对滞育的影响程度尚不清楚。为了解决这个问题，我们研究了脂肪酸合成酶(FAS)在胁迫耐受性、滞育准备时间以及昆虫是否进入滞育中的功能。在滞育准备高峰期，滞育菜蛾成虫体内FAS2的表达高于FAS1。FAS2基因敲除抑制了脂质的积累，随后影响了胁迫耐受基因的表达和水分含量。然而，沉默FAS2对滞育准备的持续时间和滞育发生率没有显著影响。保幼激素(JH)和JH受体耐甲基丁二烯(Met)抑制FAS2转录。这些结果表明，JH-Met的缺失诱导了FAS2的表达，从而促进了滞育雌性甲虫体内的脂肪储存。这些结果表明，脂肪储备调节耐逆基因的表达和水分含量，但对滞育准备的持续时间和滞育发生率没有显著影响。
Diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. Diapause-destined insects store large amounts of fat when preparing for diapause. However, the extent to which these accumulated fat reserves influence diapause remains unclear. To address this question, we investigated the function of fatty acid synthase (FAS), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause preparation, and whether insects enter diapause or not. In diapause-destined adult female cabbage beetles, Colaphellus bowringi, FAS2 was more highly expressed than FAS1 at the peak stage of diapause preparation. FAS2 knockdown suppressed lipid accumulation and subsequently affected stress tolerance genes expression and water content. However, silencing FAS2 had no significant effects on the duration of diapause preparation or the incidence of diapause. FAS2 transcription was suppressed by juvenile hormone (JH) and the JH receptor methoprene-tolerant (Met). These results suggest that the absence of JH-Met induces FAS2 expression, thereby promoting lipid storage in diapause-destined female beetles. These results demonstrate that fat reserves regulate stress tolerance genes expression and water content, but have no significant effect on the duration of diapause preparation or the incidence of diapause.