The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon.
The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon.
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DOI：
10.4161/chim.22769
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发表时间：
2012-07-01
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影响因子：
--
通讯作者：
Bianchi, Diana W
中科院分区：
文献类型：
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作者：
Pritchard, Stephanie;Peter, Inga;Bianchi, Diana W
在怀孕期间，胎儿细胞进入母体器官，并在产后居住。来自多个实验室的证据表明，这些嵌合胎儿细胞有助于损伤后母体组织的修复。在小鼠模型中，大多数损伤实验是在怀孕期间进行的；然而，在临床上，大多数伤害或疾病发生在产后。因此，使用动物模型的实验应设计为解决分娩后一段时间内的问题。为了为此类实验提供基线，我们分析了产后母体器官中胎儿细胞的自然史。雌性C57BL/6J小鼠与纯合的雄性C57BL/6J小鼠进行配对，获得增强的绿色荧光蛋白基因。利用高速流式细胞仪对母体肺和骨髓中的胎儿细胞进行绿色荧光鉴定，并比较肺和骨髓中胎儿细胞的计数。采用Spearman相关分析确定产后时间长短与细胞计数及活死细胞比之间的关系。我们的研究结果表明，在健康的雌性小鼠中，胎儿细胞在这些器官中持续存在至少三个月。我们显示了两个阶段的下降，最初两个半星期的快速清除，然后是逐渐下降的趋势。此外，随着时间的推移，肺内活细胞与死细胞比例的增加表明这些细胞可能在体内复制。这里提出的结果将为未来实验的设计提供信息，并可能对妇女的健康产生影响。
During pregnancy, fetal cells cross into the maternal organs where they reside postpartum. Evidence from multiple laboratories suggests that these microchimeric fetal cells contribute to maternal tissue repair after injury. In mouse models, most injury experiments are performed during pregnancy; however, in a clinical setting most injuries or diseases occur postpartum. Therefore, experiments using animal models should be designed to address questions in the time period following delivery. In order to provide a baseline for such experiments, we analyzed the natural history of fetal cells in the postpartum maternal organs. Female C57BL/6J mice were mated to males homozygous for the enhanced green fluorescent protein gene. Fetal cells in the maternal lungs and bone marrow were identified by their green fluorescence using in a high-speed flow cytometer and their counts were compared between the lung and bone marrow. Spearman correlation analysis was used to identify relationships between the duration of time postpartum and the cell counts and ratio of live and dead cells. Our results show that fetal cells persist in these organs until at least three months postpartum in healthy female mice. We show a two-stage decline, with an initial two and a half-week rapid clearance followed by a trend of gradual decrease. Additionally, an increase in the ratio of live to dead cells within the lung over time suggests that these cells may replicate in vivo. The results presented here will inform the design of future experiments and may have implications for women's health.