Anesthesia induces stress in Atlantic salmon (Salmo salar), Atlantic cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus)
Anesthesia induces stress in Atlantic salmon (Salmo salar), Atlantic cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus)
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DOI：
10.1007/s10695-009-9346-2
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发表时间：
2010-09-01
影响因子：
2.9
通讯作者：
Olsen, Rolf Erik
中科院分区：
文献类型：
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作者：
Zahl, Inger Hilde;Kiessling, Anders;Olsen, Rolf Erik
在大西洋鲑鱼（萨尔莫salar）、大西洋大比目鱼（Hippoglossus hippoglossus）和大西洋鳕鱼（Gadus morhua）中研究了苯并咪唑、MS-222、美咪酯和异丁香酚麻醉后的应激反应，没有与麻醉或取样相关的处理或禁闭应激反应。所有测试的麻醉剂都诱导了所有物种的应激反应，表现为皮质醇释放到水中。MS-222麻醉引起最高的皮质醇释放率，在暴露后0.5小时达到最大水平，并在3-4小时后恢复到基础水平。苯佐卡因麻醉引起双峰反应，其中皮质醇释放速率的初始峰值之后是持续到试验结束（6小时）的第二次增加。这种双峰是更深刻的大西洋鲑鱼比大西洋大比目鱼和大西洋鳕鱼。在大西洋比目鱼和大西洋鳕鱼中，美托咪酯麻醉诱导的皮质醇释放量最低，但在大西洋鲑鱼中导致双峰反应，其中皮质醇释放量最初增加，随后在暴露后2-2.5 h达到峰值，5 h后恢复到基础水平。异丁香酚麻醉大西洋鲑鱼诱导的应激与MS-222相似，但没有达到相同的升高水平。总体而言，皮质醇释放是最深刻的大西洋鲑鱼其次是大西洋大比目鱼和大西洋鳕鱼。
Stress in response to anesthesia with benzocaine, MS-222, metomidate and isoeugenol was studied in Atlantic salmon (Salmo salar), Atlantic halibut (Hippoglossus hippoglossus), and Atlantic cod (Gadus morhua) with no concomitant stress from handling or confinement in association with anesthesia or sampling. All of the anesthetics tested induced a stress response in all species, displayed by a release of cortisol to the water. MS-222 anesthesia elicited the highest cortisol release rates, reaching maximum levels 0.5 h post-exposure and returning to basal levels after 3-4 h. Benzocaine anesthesia caused a bimodal response where the initial peak in cortisol release rate was followed by a second increase lasting towards the end of the trial (6 h). This bimodality was more profound in Atlantic salmon than in Atlantic halibut and Atlantic cod. Metomidate anesthesia induced the lowest release of cortisol of the agents tested in both Atlantic halibut and Atlantic cod, but resulted in a bimodal response in Atlantic salmon where the initial increase in cortisol release was followed by a larger increase peaking at 2-2.5 h post exposure before returning to basal levels after 5 h. The stress induced in Atlantic salmon by isoeugenol anesthesia resembled that of MS-222, but did not reach the same elevated level. Overall, the cortisol release was most profound in Atlantic salmon followed by Atlantic halibut and Atlantic cod.