SCANNING ELECTRON MICROSCOPICAL STUDY OF THE INSIDE OF SEA-URCHIN EMBRYOS (PSEUDOCENTROTUS-DEPRESSUS) - EFFECTS OF ARYL BETA-XYLOSIDE, TUNICAMYCIN AND DEPRIVATION OF SULFATE-IONS
SCANNING ELECTRON MICROSCOPICAL STUDY OF THE INSIDE OF SEA-URCHIN EMBRYOS (PSEUDOCENTROTUS-DEPRESSUS) - EFFECTS OF ARYL BETA-XYLOSIDE, TUNICAMYCIN AND DEPRIVATION OF SULFATE-IONS
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DOI：
10.1016/0014-4827(80)90325-0
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
TERAYAMA, H
中科院分区：
文献类型：
--
作者：
AKASAKA, K;AMEMIYA, S;TERAYAMA, H
去除硫酸盐离子和添加对-硝基苯基-β-D-木糖苷和衣霉素可引起原肠形成的停止和骨骼形成的抑制。扫描电子显微镜(SEM)观察到胚胎内部的伪足和丝状结构的形成受到一定的损害，特别是在次生间充质细胞中，并相应地损害了间充质细胞的迁移。间充质细胞(原代和次级)或柱状细胞(囊壁)在无硫酸盐或芳基木糖苷存在时，细胞间的组织或黏附出现不同程度的损害，而在衣霉素的存在下，细胞发生碎裂。在正常的中肠胚中，次级间充质细胞位于动物极点和内陷原肠顶端之间，每个细胞的两端都有向相反方向突出的长而粗的伪足，这显然反映了原肠内陷的作用。
The deprivation of sulfate ions and the addition of p-nitrophenyl .beta.-D-xyloside and tunicamycin gave rise to the arrest of gastrulation and the inhibition of skeletal formation in Pseudocentrotus embryos. Scanning electron microscopical (SEM) observations of the inside of embryos revealed some impairment of the formation of pseudopodia and filamentous structures, especially in the secondary mesenchyme cells, and accordingly of the migration of the mesenchyme cells. The intercellular organization or adhesion of mesenchyme cells (primary and secondary) or columnar cells (in the blastular wall) appeared more or less impaired in the absence of sulfate or the presence of aryl xyloside, and cellular fragmentation was observed in the presence of Tunicamycin. In the normal mid-gastrula the secondary mesenchyme cells were localized between the animal pole and the tip of invaginating archenteron, and long, thick pseudopodia protruding from the 2 ends of each cell in opposite directions were observed, apparently reflecting a role in the invagination of archenteron.