Platelet-dependent thrombin generation in patients with hyperlipidemia
Platelet-dependent thrombin generation in patients with hyperlipidemia
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DOI：
10.1016/s0735-1097(97)00129-0
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发表时间：
1997-07-01
影响因子：
24
通讯作者：
Ishikawa, K
中科院分区：
文献类型：
--
作者：
Aoki, I;Aoki, N;Ishikawa, K
目标.我们通过测定血小板依赖性凝血酶的生成来评估高胆固醇血症患者在普伐他汀治疗前后以及高胆固醇血症患者的凝血功能，以研究凝血功能作为动脉粥样硬化指标的有用性，并确定治疗高胆固醇血症的重要性。了解血小板与血浆凝血系统之间的相互作用对于阐明促凝血过程的机制是重要的。我们评估了58例高胆固醇血症患者的凝血功能（血清总胆固醇水平≥ 220 mg/dl，年龄56.5 ± 1.5岁[平均值± SEM]），37例高胆固醇血症患者（血清甘油三酯水平大于或等于200 mg/dl，年龄59.5 ± 1.7岁），13例高胆固醇血症伴高胆固醇血症患者（年龄51.4 ± 3.1岁）和75例正常受试者（年龄52.2 ± 1.7岁）。我们还研究了高胆固醇血症患者在普伐他汀治疗前后血小板依赖性凝血酶的生成。将氯化钙添加到0.5 ml富血小板血浆（150 x 10（9）/升）中以引发凝血。每隔10分钟将10 μ l样品转移至90 μ l 3.8%柠檬酸钠中30分钟。向每个样品中加入显色底物S-2238，并在405 nm波长下用比色法测量吸光度，以确定凝血酶生成。与高胆固醇血症患者和对照组相比，高胆固醇血症患者和高胆固醇血症合并高胆固醇血症患者的血小板依赖性凝血酶生成增加（p < 0.01）。普伐他汀治疗使凝血酶生成正常化。高胆固醇血症与血小板依赖性凝血酶生成增加相关，但高胆固醇血症与血小板依赖性凝血酶生成增加无关。普伐他汀使凝血酶的生成正常化。(C)1997年，美国心脏病学会。
Objectives. We evaluated coagulability as determined by platelet-dependent thrombin generation in hypercholesterolemic patients before and after treatment with pravastatin and in hypertriglyceridemic patients to investigate the usefulness of coagulability as an index of atherosclerosis and to determine the importance of treating hyperlipidemia.Background. An understanding of the interaction between platelets and the plasma coagulation system is important for clarifying the mechanism of the procoagulant process.Methods. We assessed coagulability in 58 patients with hypercholesterolemia (serum total cholesterol level greater than or equal to 220 mg/dl, age 56.5 +/- 1.5 years [mean +/- SEM]), 37 patients with hypertriglyceridemia (serum triglyceride level greater than or equal to 200 mg/dl, age 59.5 +/- 1.7 years), 13 patients with hypercholesterolemia plus hypertriglyceridemia (age 51.4 +/- 3.1 years) and 75 normal subjects (age 52.2 +/- 1.7 years). We also studied platelet-dependent thrombin generation in patients with hypercholesterolemia before and after treatment with pravastatin. Calcium chloride was added to 0.5 mi of platelet-rich plasma (150 x 10(9)/liter) to initiate coagulation. Ten microliters of the sample was transferred into 90 mu l of 3.8% sodium citrate at 10-min intervals for 30 min. A chromogenic substrate, S-2238, nas added to each sample, and absorbance was measured spectrophotometrically at a wavelength of 405 nm to determine thrombin generation.Results. Platelet-dependent thrombin generation was increased in patients with hypercholesterolemia and patients with hypercholesterolemia plus hypertriglyceridemia (p < 0.01) compared with patients with hypertriglyceridemia and control subjects. Treatment with pravastatin normalized thrombin generation.Conclusions. Hypercholesterolemia, but not hypertriglyceridemia, was associated with increased platelet-dependent thrombin generation. Pravastatin normalized the generation of thrombin. (C) 1997 by the American College of Cardiology.