Resonance Raman investigation of a soluble cytochrome c552 from alkaliphilic Bacillus firmus RAB.
Resonance Raman investigation of a soluble cytochrome c552 from alkaliphilic Bacillus firmus RAB.
复制标题
对来自嗜碱性芽孢杆菌 RAB 的可溶性细胞色素 c552 进行共振拉曼研究。
DOI：
10.1016/0003-9861(90)90641-b
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发表时间：
1990
影响因子：
3.9
通讯作者：
Ondrias,MR
中科院分区：
文献类型：
--
作者：
Larsen,RW;Chavez,MD;Nunez,DJ;Davidson,MW;Knaff,DB;Krulwich,TA;Ondrias,MR
用共振拉曼散射对来自嗜碱杆菌firmusRAB的低电位可溶性细胞色素（c552）的血红素位点进行了表征，并与马心脏细胞色素进行了比较。拉曼数据表明，细胞色素552中对血红素轴向结扎敏感的振动带以及对血红素周围环境敏感的模式与马心脏细胞色素不同。细胞色素552的光谱显示共振拉曼模式，表明氧化态的第六配体是蛋氨酸，而还原态的第六配体似乎含有氮基的第六配体。此外，q波段激发揭示了细胞色素552中对血红素周围氨基酸环境敏感的振动模式的差异。
The environment of the heme site of a low-potential soluble cytochrome (c552) from alkaliphilicBacillus firmusRAB has been characterized with resonance Raman scattering and compared to that of horse heart cytochromec. The Raman data indicate that vibrational bands sensitive to the axial ligation of the heme, as well as modes sensitive to the heme peripheral environment in cytochromec552, are distinct from those of horse heart cytochromec. The spectra of cytochromec552display resonance Raman modes indicative of a methionine as the sixth ligand in the oxidized form, while the reduced form appears to contain a nitrogenous-based sixth ligand. In addition, Q-band excitation reveals differences among vibrational modes in cytochromec552that are sensitive to the amino acid environment surrounding the heme.