Determination of in vitro synergy when three antimicrobial agents are combined against Mycobacterium tuberculosis
Determination of in vitro synergy when three antimicrobial agents are combined against Mycobacterium tuberculosis
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DOI：
10.1016/j.ijantimicag.2005.05.005
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发表时间：
2005-10-01
影响因子：
10.8
通讯作者：
Yamane, N
中科院分区：
文献类型：
--
作者：
Bhusal, Y;Shiohira, CM;Yamane, N
我们使用三维棋盘在Middlebrook 7H9肉汤微量稀释液中测定了利福平、异烟肼和第三种药物组合的体外抗分枝杆菌活性。在筛选的28种药物中，乙胺丁醇、链霉素、克拉霉素、米诺环素、环丙沙星、左氧氟沙星、司帕沙星、加替沙星和西他沙星具有潜在的协同作用。进一步的三维棋盘测定定量地寻找对结核分枝杆菌的10个临床分离株的协同作用，包括7个多药耐药分离株。西他沙星、加替沙星和克拉霉素显示出显著的协同作用，其分数抑制浓度指数分别为0.41至0.79、0.39至0.90和0.48至0.95。结论：三维棋盘法可以定量测定抗分枝杆菌协同作用，氟喹诺酮类和克拉霉素等抗菌药物对多重耐药分枝杆菌有效。结核病与利福平和异烟肼联合使用。(c)2005年Elsevier B.V.和国际化疗学会。All rights reserved.
We determined the in vitro antimycobacterial activity of rifampicin, isoniazid and a third agent in combination using a three-dimensional chequerboard in Middlebrook 7H9 broth microdilutions. Of 28 agents screened, ethambutol, streptomycin, clarithromycin, minocycline, ciprofloxacin, levofloxacin, sparfloxacin, gatifloxacin and sitafloxacin were potentially synergistic. A further three-dimensional chequerboard assay quantitatively looked for synergy against ten clinical isolates of Mycobacterium tuberculosis, including seven multidrug-resistant isolates. Sitafloxacin, gatifloxacin and clarithromycin showed significant synergy, with fractional inhibitory concentration indices ranging from 0.41 to 0.79, 0.39 to 0.90 and 0.48 to 0.95, respectively. It is concluded that three-dimensional chequerboard assay can quantitatively determine antimycobacterial synergy, and that fluoroquinolones and antibacterial agents such as clarithromycin are effective against multidrug-resistant isolates of M. tuberculosis when combined with rifampicin and isoniazid. (c) 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.