Imaging Early Demineralization on Tooth Occlusal Surfaces with a High Definition InGaAs Camera.
Imaging Early Demineralization on Tooth Occlusal Surfaces with a High Definition InGaAs Camera.
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DOI：
10.1117/12.2011015
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发表时间：
2013-03-25
期刊：
影响因子：
--
通讯作者：
Darling CL
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文献类型：
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作者：
Fried WA;Fried D;Chan KH;Darling CL
体内和体外研究表明，由于牙釉质的高透明度，可以在近红外中获得牙齿脱矿的高对比度图像。本研究的目的是将与高吸水率一致的近红外波长下的病变反射率与可见光、1300 nm 近红外波长下的病变对比度以及咬合面早期病变的荧光测量值进行比较。这项体外研究使用了二十四颗人类臼齿。牙齿涂有耐酸清漆，在每颗牙齿的咬合面留下一个 4×4 毫米的窗口，以进行脱矿质。暴露于 pH 4.5 的脱矿质溶液 1 天和 2 天后，暴露的窗户产生人工损伤。使用高清 InGaAs 相机，使用 1310、1460 和 1600 nm 3 个波长的近红外反射率对病变进行成像。还使用可见光反射率以及 405 nm 激发和波长大于 500 nm 检测的荧光来获取图像以进行比较。交叉偏振器用于反射率测量，以减少镜面反射率的干扰。 1460 nm 和 1600 nm 的 NIR 反射成像的 24 小时和 48 小时病变的对比度显着高于 1300 nm 的 NIR 反射成像、可见光反射成像和荧光。这项研究的结果表明，较长近红外波长下的近红外反射率测量与较高的吸水率一致，更适合对早期龋齿病变进行成像。
In vivo and in vitro studies have shown that high contrast images of tooth demineralization can be acquired in the near-IR due to the high transparency of dental enamel. The purpose of this study is to compare the lesion contrast in reflectance at near-IR wavelengths coincident with high water absorption with those in the visible, the near-IR at 1300-nm and with fluorescence measurements for early lesions in occlusal surfaces. Twenty-four human molars were used in this in vitro study. Teeth were painted with an acid-resistant varnish, leaving a 4×4 mm window in the occlusal surface of each tooth exposed for demineralization. Artificial lesions were produced in the exposed windows after 1 & 2-day exposure to a demineralizing solution at pH 4.5. Lesions were imaged using NIR reflectance at 3 wavelengths, 1310, 1460 and 1600-nm using a high definition InGaAs camera. Visible light reflectance, and fluorescence with 405-nm excitation and detection at wavelengths greater than 500-nm were also used to acquire images for comparison. Crossed polarizers were used for reflectance measurements to reduce interference from specular reflectance. The contrast of both the 24 hr and 48 hr lesions were significantly higher (P<0.05) for NIR reflectance imaging at 1460-nm and 1600-nm than it was for NIR reflectance imaging at 1300-nm, visible reflectance imaging, and fluorescence. The results of this study suggest that NIR reflectance measurements at longer near-IR wavelengths coincident with higher water absorption are better suited for imaging early caries lesions.