Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis
Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis
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DOI：
10.1038/sj.emboj.7601637
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发表时间：
2007-03-21
期刊：
影响因子：
11.4
通讯作者：
Hall, Alan
中科院分区：
文献类型：
--
作者：
Schmidt, Anja;Durgan, Joanne;Hall, Alan
Rho GTP酶调节多种信号转导途径，这些途径影响细胞行为的许多方面，包括迁移、形态、极性和细胞周期。通过它们控制肌动蛋白和微管细胞骨架的组装和组织的能力，Rho和Cdc 42在细胞周期的有丝分裂阶段做出几个关键贡献，包括纺锤体组装、纺锤体定位、卵裂沟收缩和分裂。我们现在报告PRK 2/PKN 2，一个Ser/Thr激酶和Rho/Rac效应蛋白，是HeLa S3细胞进入有丝分裂和退出胞质分裂的重要调节因子。PRK 2是细胞分裂周期结束时中间体的分解和Cdc 25 B的磷酸化和活化所必需的，Cdc 25 B是在G2/M转换时活化有丝分裂细胞周期蛋白/Cdk 1复合物所需的磷酸酶。这揭示了哺乳动物细胞周期中由Rho GTP酶控制的额外步骤。
\ Rho GTPases regulate multiple signal transduction pathways that influence many aspects of cell behaviour, including migration, morphology, polarity and cell cycle. Through their ability to control the assembly and organization of the actin and microtubule cytoskeletons, Rho and Cdc42 make several key contributions during the mitotic phase of the cell cycle, including spindle assembly, spindle positioning, cleavage furrow contraction and abscission. We now report that PRK2/PKN2, a Ser/Thr kinase and Rho/Rac effector protein, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells. PRK2 is required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B, the phosphatase required for activation of mitotic cyclin/Cdk1 complexes at the G2/M transition. This reveals an additional step in the mammalian cell cycle controlled by Rho GTPases.