In vivo and in vitro analysis of the vasculogenic potential of avian proepicardial and epicardial cells
In vivo and in vitro analysis of the vasculogenic potential of avian proepicardial and epicardial cells
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DOI：
10.1002/dvdy.20685
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发表时间：
2006-04-01
影响因子：
2.5
通讯作者：
Pérez-Pomares, JM
中科院分区：
文献类型：
--
作者：
Guadix, JA;Carmona, R;Pérez-Pomares, JM
在胚胎血管发育的背景下，冠状血管的形成是一种特殊情况。冠状细胞前体（内皮细胞、平滑肌细胞和成纤维细胞）的主要部分来源于心外膜原基和心外膜，这可被视为成血管细胞和平滑肌细胞分化的晚期事件。因此，冠状血管形态发生依赖于心外膜原基和心外膜的上皮 - 间质转化。在这项研究中，我们对禽类胚胎冠状血管发生过程提出了一些新的观察结果，即：（1）心外膜原基在体内（如异位移植所示）和体外都显示出很高的血管发生潜能，这种潜能受血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子信号的调节；（2）心外膜原基细胞和心外膜细胞共同表达血小板衍生生长因子 - BB和VEGF的受体；（3）冠状成血管细胞（遍布心外膜、心外膜下和致密心肌层）表达威尔姆斯瘤相关转录因子和视黄酸合成酶视黄醛脱氢酶 - 2，这是参与冠状内皮发育的体腔上皮的两种标志物。所有这些结果都有助于增进我们对心外膜原基/心外膜细胞的血管潜能、分化中的冠状细胞谱系之间现有的相互关系以及参与冠状血管形态发生调节的分子机制的认识。
Coronary vessel formation is a special case in the context of embryonic vascular development. A major part of the coronary cellular precursors (endothelial, smooth muscle, and fibroblastic cells) derive from the proepicardium and the epicardium in what can be regarded as a late event of angioblastic and smooth muscle cell differentiation. Thus, coronary morphogenesis is dependent on the epithelial-mesenchymal transformation of the proepicardium and the epicardium. In this study, we present several novel observations about the process of coronary vasculogenesis in avian embryos, namely: (1) The proepicardium displays a high vasculogenic potential, both in vivo (as shown by heterotopic transplants) and in vitro, which is modulated by vascular endothelial growth factor (VEGF) and basic fibroblast growth factor signals; (2) Proepicardial and epicardial cells co-express receptors for platelet-derived growth factor-BB and VEGF; (3) Coronary angioblasts (found all through the epicardial, subepicardial, and compact myocardial layers) express the Wilms' tumor associated transcription factor and the retinoic acid-synthesizing enzyme retinaldehyde-dehydrogenase-2, two markers of the coelomic epithelium involved in coronary endothelium development. All these results contribute to the development of our knowledge on the vascular potential of proepicardiallepicardial cells, the existent interrelationships between the differentiating coronary cell lineages, and the molecular mechanisms involved in the regulation of coronary morphogenesis.