Alveolar bone loss and mineralization in the pig with experimental periodontal disease.
Alveolar bone loss and mineralization in the pig with experimental periodontal disease.
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DOI：
10.1016/j.heliyon.2018.e00589
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发表时间：
2018-03
期刊：
影响因子：
4
通讯作者：
Liu ZJ
中科院分区：
文献类型：
--
作者：
Yang M;Nam GE;Salamati A;Baldwin M;Deng M;Liu ZJ
探讨实验性牙周病如何影响幼猪模型中的牙槽骨质量和矿物质沉积。七只三个月大的猪定期接种 4 种牙周细菌，并在最后一颗上颌乳磨牙周围结扎 8 周，以诱发牙周病 (PG)。八只同龄猪作为对照（CG）。对 3D 锥形束 CT 图像进行分割，以量化总牙槽骨、牙槽嵴和目标磨牙所有牙根的体积。安乐死前给予钙黄绿素和茜素以标记矿物质并置。将收获的磨牙块切片并在落射荧光下检查。测量区域骨表面两个生命标记之间的标签间距离并计算矿物质沉积率（MAR）。 PG 中总牙槽骨体积显着减少，其中牙槽嵴主要损失。 CG 中，根分叉区域的 MAR 显着高于颊侧和腭侧的区域。与 CG 相比，PG 动物在分叉区域显示出更多中断的标记带，且 MAR 显着降低。 MAR 与 CG 中的总牙槽骨和牙槽嵴体积呈正相关，但仅与 PG 中的总牙槽骨体积呈正相关。在生长中的幼猪中，矿物质的添加具有地区特异性。实验性牙周病不仅导致牙槽骨流失，还会扰乱新骨形成的矿物质沉积，从而损害牙槽骨重塑的稳态。
To address how experimental periodontal disease affects alveolar bone mass and mineral apposition in a young pig model. Seven three-month-old pigs were periodically inoculated with 4 types of periodontal bacteria, along with a ligature around the last maxillary deciduous molar for 8 weeks to induce periodontal disease (PG). Eight same-aged pigs served as the control (CG). Segmentations of 3D cone-beam CT images were performed to quantify volumes of the total alveolar bone, alveolar ridge, and all roots of the target molar. Calcein and alizarin were administered for labeling mineral apposition before euthanasia. The harvested molar blocks were sectioned and examined under epifluorescence. The inter-label distance between the two vital markers at regional bone surfaces were measured and mineral apposition rate (MAR) was calculated. A significant reduction of total alveolar bone volume was seen in PG with the major loss at the alveolar ridge. MAR was significantly higher at the root furcation region than those at both buccal and palatal ridges in CG. Compared with CG, PG animals showed more interrupted labeled bands with significantly lower MAR at the furcation region. MARs were positively associated with both the volumes of total alveolar bone and ridge in CG, but only with the total alveolar bone in PG. In young growing pigs, mineral apposition is region specific. The experimental periodontal disease not only leads to alveolar bone loss, but also perturbs mineral apposition for new bone formation, thus impairing the homeostasis of alveolar bone remodeling.