Interleukin-18 in plasma and adipose tissue: effects of obesity, insulin resistance, and weight loss
Interleukin-18 in plasma and adipose tissue: effects of obesity, insulin resistance, and weight loss
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DOI：
10.1530/eje-07-0206
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发表时间：
2007-10-01
影响因子：
5.8
通讯作者：
Richelsen, Bjorn
中科院分区：
文献类型：
--
作者：
Bruun, Jens M.;Stallknecht, Bente;Richelsen, Bjorn
目的：白细胞介素18(IL-18)与肥胖、胰岛素抵抗有关。和心血管疾病。本研究比较了1)脂肪细胞和间质血管(SV)细胞中的IL-18，2)肥胖者血浆和脂肪组织(AT)中的IL-18，以及3)血浆中的IL-18。研究对象和方法：对23例肥胖者和12例瘦肉者在基线状态下血浆和AT IL-18水平进行比较。肥胖受试者接受为期15周的生活方式干预(低热量饮食和日常锻炼)，之后采集血浆样本，AT。并进行SM活检。结果：IL-18在分离的脂肪细胞中的表达与SV细胞中IL-18的表达水平相近。肥胖患者血浆IL-18水平较高(P<；0.001)，且与胰岛素抵抗(HOMA；P<；0.001)相关。IL-18、CD14、CD68在肥胖组的表达高于肥胖组(P<0.01)。干预降低了体重(P<；0.001)，降低了血浆IL-18(P<；0.001)，提高了胰岛素敏感性(P<；0.05)。干预后IL-18的AT和SM表达无明显变化。血浆IL-18水平变化与胰岛素敏感性改变相关(P<；0.05)，与体重指数、IL-18 AT表达无关。结论：血浆IL-18水平与胰岛素抵抗有关，减肥后血浆IL-18水平降低。IL-18在肥胖时表达增加，但不受体重减轻的影响，提示血浆IL-18的变化与胰岛素抵抗有关，而不是肥胖本身的变化。
Objective: Interleukin (IL)-18 is associated with obesity, insulin resistance. and cardiovascular disease. The present study compared 1) IL-18 in adipocytes versus stromal vascular (SV) cells, 2) IL-18 in plasma and adipose tissue (AT) in obese versus lean subjects, and 3) IL-18 in plasma. AT, and skeletal muscle (SM) in obese subjects after weight loss.Subjects and methods: At baseline, plasma and AT IL-18 in 23 obese subjects were compared with that in 12 lean subjects. The obese subjects were submitted to a 15-week life-style intervention (hypocaloric diet and daily exercise) after which plasma samples, AT. and SM biopsies were obtained. Analyses were performed by ELISA and RT-PCR respectively.Results: IL-18 expression in isolated adipocytes was similar to 2%, of that in SV cells. Plasma IL-18 was higher in obese subjects (P < 0.001) and associated with insulin resistance (HOMA; P < 0.001). AT expression of IL-18, CD14, and CD68 was higher in obese (P < 0.01). The intervention reduced body weight (P < 0.001), plasma IL-18 (P < 0.001), and increased insulin sensitivity (HOMA; P < 0.05). AT and SM expression of IL-18 remained unchanged after the intervention. Changes in plasma IL-18 were associated with changes in insulin sensitivity (P < 0.05) but not with BMI or AT expression of IL-18.Conclusion:Plasmal IL-18 is associated with changes in insulin resistance and reduced after weight loss. AT expression of IL-18 is increased in obesity but not affected by weight loss, indicating that changes in plasma IL-18 are related to insulin resistance rather than changes in obesity per se.