Antifungal Activity of Crude Extract from the Rhizome and Root of Smilacina japonica A. Gray
Antifungal Activity of Crude Extract from the Rhizome and Root of Smilacina japonica A. Gray
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菝葜根茎粗提物的抗真菌活性
DOI：
10.1155/2019/5320203
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发表时间：
2019
影响因子：
--
通讯作者：
Wang Shengzheng
中科院分区：
文献类型：
--
作者：
Liu Wei;Sun Baozheng;Yang Manman;Zhang Ziyue;Zhang Xiuxiu;Pang Tingsong;Wang Shengzheng
研究鹿药水醇提取物的体外抗真菌活性。Gray（SJA）对不同真菌的抗性。采用微量肉汤稀释法测定SJA的最低抑菌浓度（MIC）。细胞生长试验和时间-杀灭曲线试验进一步证实了SJA对白色念珠菌的抑菌作用。并研究了SJA对真菌形态和超微结构的影响。SJA具有广谱抗真菌活性。SJA对不同真菌的MIC范围为208 μg/ml至1665 μg/ml，包括对氟康唑敏感和耐药的白色念珠菌、其他念珠菌属和新型隐球菌。此外，SJA对多种真菌具有杀菌活性，并明显抑制真菌菌丝生长。其抗真菌作用机制可能与其对细胞形态和超微结构的影响有关。
This study aimed to investigate the antifungal activity of hydroalcoholic extract from Smilacina japonica A. Gray (SJA) against different fungi. The minimum inhibitory concentration (MIC) for SJA was determined by the broth microdilution method. The antifungal effects of SJA against Candida albicans were further confirmed by cell growth test and time-kill curve test. The effects of SJA on the fungal morphology and ultrastructure were also evaluated. SJA has a broad-spectrum antifungal activity. The MICs of SJA against different fungi, including fluconazole-sensitive and -resistant Candida albicans, other Candida species, and Cryptococcus neoformans, ranged from 208 μg/ml to 1665 μg/ml. Furthermore, SJA displayed fungicidal activity against varied fungi and obviously inhibited the hyphal growth of fungi. The mechanism study revealed that the antifungal activity of SJA might be associated with its effect on the cell morphology and ultrastructure.