Ionic-liquid matrices for quantitative analysis by MALDI-TOF mass spectrometry
Ionic-liquid matrices for quantitative analysis by MALDI-TOF mass spectrometry
复制标题
DOI：
10.1016/j.jasms.2004.08.011
复制
发表时间：
2004-12
影响因子：
3.2
通讯作者：
Ying L. Li;M. Gross
中科院分区：
文献类型：
--
作者：
Ying L. Li;M. Gross
离子液体基质（ILM）作为MALDI基质进行了测试，用于定量寡脱氧核苷酸（ODN），肽和小蛋白质。尽管它们的物理状态不同，但在所有测试的ILM的宽浓度范围内实现了具有高线性和再现性的良好校准。然而，对于具有可见晶体的固态ILM，标准偏差较高。实验结果表明，不同的离子膜由于其阳离子组分的变化而具有不同的灵敏度。更重要的是，我们发现，校准曲线的斜率与肽分子量的倒数相关，提供了一个机会来预测先验的相对灵敏度（校准曲线的斜率），具有类似的疏水性的各种分析物。
Ionic liquid matrices (ILMs) were tested as MALDI matrices for quantification of oligodeoxynucleotides (ODNs), peptides, and small proteins. Good calibrations with high linearity and reproducibility were achieved over a broad concentration range for all the tested ILMs in spite of their different physical states. However, the standard deviation is higher for ILMs that are solid with visible crystals. The experimental results indicate various ILMs have different sensitivity owing to changes in their cation components. More importantly, we found that the slopes of the calibration curves correlate with the inverse of the peptide molecular weights, presenting an opportunity to predict a priori, the relative sensitivities (slopes of calibration plots) for various analytes that have similar hydrophobicites.