The effects of heterologous liver cross-hemodialysis on adenylate energy charge of the remnant liver after major hepatic resection.
The effects of heterologous liver cross-hemodialysis on adenylate energy charge of the remnant liver after major hepatic resection.
复制标题
异源肝脏交叉血液透析对主要肝切除术后残余肝脏腺苷酸能量电荷的影响。
DOI：
10.1111/j.1525-1594.1982.tb04142.x
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发表时间：
1982
影响因子：
2.4
通讯作者：
T. Tobe
中科院分区：
文献类型：
--
作者：
K. Ozawa;Y. Kamiyama;K. Kimura;M. Ukikusa;Y. Kono;T. Yamato;Y. Shimahara;T. Nakatani;M. Asano;R. Irie;S. Kawashima;K. Uchida;M. Ohtoshi;H. Aoyama;F. Hirai;K. Yasuda;T. Tobe
在兔子身上研究了肝脏支持对主要肝切除术后残余肝脏腺苷酸能量电荷（ATP + 0.5ADP）/（ATP + ADP + AMP）的影响。本研究强调了通过使用插入Cuprophan膜的离体异源肝脏交叉血液透析来恢复主要肝切除后残余肝脏降低的能量电荷水平的原理。能量电荷水平为细胞提供了非常敏感的细胞内控制机制。来自生物合成序列的调节酶在低能量水平下表现出非常小的活性，并且它们的活性在高能量水平下急剧增加。 70%肝切除术后24小时，残余肝脏的能量电荷水平从对照水平0.860最大降低至0.767。在 24 小时 70% 肝切除的兔子和具有高能量电荷的离体猪肝之间插入 Cuprophan 膜进行交叉血液透析两个小时后，能量电荷水平从 0.767 增加到 0.857。上述结果表明，这种离体猪交叉血液透析可能对残肝再生过程中的生物合成有效。
The effect of liver support on the adenylate energy charge (ATP + 0.5ADP)/(ATP + ADP + AMP) of the remnant liver after major hepatic resection was studied in rabbits. The present study emphasized the principle of restoring the decreased energy charge level of the remnant liver after major hepatic resection by use of an ex vivo heterologous liver cross-hemodialysis with an interposed Cuprophan membrane. The energy charge level provides the cell with a very sensitive intracellular control mechanism. Regulatory enzymes from biosynthetic sequences exhibit very little activity at low levels of energy charge, and their activities increase sharply at high-energy charge levels. The energy charge level of the remnant liver maximally decreased from the control level of 0.860 to 0.767 at 24 hours after 70% hepatectomy. The energy charge level increased from 0.767 to 0.857 after two hours of cross-hemodialysis with an interposed Cuprophan membrane between the 24-hour, 70% hepatectomized rabbit and an ex vivo pig liver with high energy charge. The above results suggest that this ex vivo pig cross-hemodialysis may be effective for biosynthesis in the regenerative processes of the remnant liver.