"Bronchial artery delivery of viral vectors for gene delivery in cystic fibrosis; superior to airway delivery?".
"Bronchial artery delivery of viral vectors for gene delivery in cystic fibrosis; superior to airway delivery?".
复制标题
“病毒载体的支气管动脉在囊性纤维化中输送基因递送；优于气道输送？”。
DOI：
10.1186/1471-2466-2-2
复制
发表时间：
2002-04-03
影响因子：
3.1
通讯作者：
Coutelle CC
中科院分区：
文献类型：
--
作者：
Bakhai A;Sheridan DJ;Coutelle CC
针对囊性纤维化肺部表现的基因治疗尝试主要依赖于气道输送。然而，气道上皮细胞中基因转移和表达的效率尚未达到治疗水平。由于多种原因，上皮细胞的通路并不均匀，并且不能通过气道到达粘膜下腺体。我们建议将基因递送载体直接注射到支气管动脉中，并结合预递送血管内皮生长因子，以增加血管内皮通透性，并通过球囊闭塞减少递送后的流量。因此，有可能以增加且同质的方式到达支气管腔上皮和粘膜下腺的粘液分泌细胞。据我们所知，这种技术组合以前尚未被研究过，并且可能使我们能够克服目前囊性纤维化基因治疗的一些限制。
Attempts at gene therapy for the pulmonary manifestations of Cystic Fibrosis have relied mainly on airway delivery. However the efficiency of gene transfer and expression in the airway epithelia has not reached therapeutic levels. Access to epithelial cells is not homogenous for a number of reasons and the submucosal glands cannot be reached via the airways. We propose to inject gene delivery vectors directly into bronchial arteries combined with pre-delivery of vascular endothelial growth factor to increase vascular endothelial permeability and post-delivery flow reduction by balloon occlusion. Thus it may be possible to reach mucous secreting cells of the bronchial luminal epithelium and the submucosal glands in an increased and homogenous fashion. This combination of techniques to the best of our knowledge has not previously been investigated, and may enable us to overcome some of the current limitations to gene therapy for Cystic Fibrosis.