Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats
Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats
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DOI：
10.1038/s41596-021-00539-9
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发表时间：
2021-06
期刊：
影响因子：
14.8
通讯作者：
Rik J J van Daal-Rik-J-J-van-Daal-2007891459;C. Aydın;Frédéric Michon;A. Aarts;M. Kraft;F. Kloosterman;S. Haesler
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作者：
Rik J J van Daal-Rik-J-J-van-Daal-2007891459;C. Aydın;Frédéric Michon;A. Aarts;M. Kraft;F. Kloosterman;S. Haesler
神经回路的动态活动如何引起行为是神经科学的一个主要研究领域。解决这个问题的一个关键实验方法是测量清醒、行为正常的动物的细胞外神经元活动。最近开发的神经像素探针在记录高时空分辨率的大组织体积的神经活动方面提供了一个步骤变化。该方案描述了神经像素探针在小鼠和大鼠使用紧凑和可重复使用的3d打印装置的慢性植入。该装置有助于在自由行为的大鼠和小鼠体内进行稳定的慢性记录。它们由两部分组成：一个覆盖的主体和一个头骨连接器。单，双和移动探针夹具变体是可用的。实验完成后，探针通过专用的回收机构安全回收以重新植入。夹具组装和手术植入通常需要4-5小时，探针回收需要~30分钟，然后在探针清洗剂中孵育12小时。数据采集的持续时间取决于行为实验的类型。由于我们的方案能够在数周内实现稳定、长期的记录，因此它能够以经济有效的方式常规获得纵向大规模单单元数据，这将促进系统神经科学的许多研究。
How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently developed Neuropixels probes have provided a step change in recording neural activity in large tissue volumes with high spatiotemporal resolution. This protocol describes the chronic implantation of Neuropixels probes in mice and rats using compact and reusable 3D-printed fixtures. The fixtures facilitate stable chronic in vivo recordings in freely behaving rats and mice. They consist of two parts: a covered main body and a skull connector. Single-, dual- and movable-probe fixture variants are available. After completing an experiment, probes are safely recovered for reimplantation by a dedicated retrieval mechanism. Fixture assembly and surgical implantation typically take 4–5 h, and probe retrieval takes ~30 min, followed by 12 h of incubation in probe cleaning agent. The duration of data acquisition depends on the type of behavioral experiment. Since our protocol enables stable, chronic recordings over weeks, it enables longitudinal large-scale single-unit data to be routinely obtained in a cost-efficient manner, which will facilitate many studies in systems neuroscience.