Trypanosoma amblyommi sp. nov. (Protozoa: Kinetoplastida) isolated from Amblyomma brasiliense (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil
Trypanosoma amblyommi sp. nov. (Protozoa: Kinetoplastida) isolated from Amblyomma brasiliense (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil
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温和锥虫 sp.
DOI：
10.1017/pao.2018.6
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Marotta C
中科院分区：
文献类型：
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作者：
Marotta C
锥虫属的寄生虫是显示出广泛的形态、生物学和遗传变异性的微生物。在这里，我们提出了第一个描述的属锥虫自然感染蜱Amblyomma brasiliense分离。从巴西里约热内卢Itatiaia市Itatiaia国家公园的Tayassu pecari（Queixada，白唇野猪）标本中采集蜱虫。分离物的特征在于分子，形态和生物学分析。从压碎的若虫和成虫蜱中分离锥虫属培养物，在蜱细胞系IDE 8中繁殖，并在L15 B培养基中维持，在32 ℃下孵育。该分离物在30、32和34 °C的L15B培养基中生长良好，但在更低或更高的温度下生长不好。培养物在30 °C下在无菌L15B培养基中保持稳定。冷冻保存的培养物在液氮中冷冻保存后保持活力。分析了锥虫在无菌培养基中的生长和发育形式。18S rDNA序列分析证实了该新种的真实性，并将其核苷酸序列存入Genbank。该物种被命名为锥虫Amblyommi sp. nov.菌株C1RJ。与致病性有关的特征，涉及脊椎动物宿主，流行病学，发育周期和传播机制仍然未知。因此，有必要进一步研究T.钝眼蜱新种
Parasites of the genus Trypanosoma are microorganisms that display wide morphological, biological and genetic variability. Here we present the first description of an isolate of the genus Trypanosoma naturally infecting the tick Amblyomma brasiliense. The ticks were collected from a specimen of Tayassu pecari (Queixada, white-lipped peccary) from the Itatiaia National Park, Itatiaia, Rio de Janeiro, Brazil. The isolate was characterized by molecular, morphometric and biological analyses. A Trypanosoma culture was isolated from crushed nymphal and adult ticks, propagated in the tick cell line IDE8 and maintained in L15B culture medium, incubated at 32 °C. The isolate grew well in L15B medium at 30, 32 and 34 °C but not at lower or higher temperatures. The culture remained stable in axenic L15B medium at 30 °C. Cryopreserved cultures retained viability after cryopreservation in liquid nitrogen. Growth in axenic medium and developmental forms of the trypanosomes were analysed. Analysis of the 18S rDNA region confirmed the authenticity of this new species and the nucleotide sequence was deposited in Genbank. The species was named Trypanosoma amblyommi sp. nov. strain C1RJ. Characteristics related to pathogenicity, involvement with vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms are still unknown. Therefore, further studies are necessary to understand the aspects of the biological cycle of T. amblyommi sp. nov.