In silico prediction of the T-cell and IgE-binding epitopes of Per a 6 and Bla g 6 allergens in cockroaches
In silico prediction of the T-cell and IgE-binding epitopes of Per a 6 and Bla g 6 allergens in cockroaches
复制标题
蟑螂 Per a 6 和 Bla g 6 过敏原的 T 细胞和 IgE 结合表位的计算机预测
DOI：
10.3892/mmr.2014.2399
复制
发表时间：
2014-10-01
影响因子：
3.4
通讯作者：
Tao, Ai-Lin
中科院分区：
文献类型：
--
作者：
Chen, Hao;Yang, Hai-Wei;Tao, Ai-Lin
Pera 6和Blag 6是分别在美洲大蠊和德国小蠊中发现的蟑螂过敏原。本研究的目的是预测Per a 6和Bla g 6变应原的B-和T-细胞表位。使用DNAStar Protean系统、Bioinformatics Predicted Antigenic Peptides系统和BepiPred 1.0服务器3种免疫信息学工具预测潜在的B细胞表位，而使用Net-MHCIIpan-2.0和NetMHCII-2.2预测两种过敏原的T细胞表位。结果，在B细胞表位预测中，在Per α 6过敏原中预测了7种肽，在Bla α 6过敏原中预测了7种肽。在T细胞预测中，预测Per α 6变应原具有9个强结合的九聚体核心表位序列（IC 50
Per a 6 and Bla g 6 are cockroach allergens found in Periplaneta americana and Blattella germanica, respectively. The objective of the present study was to predict the B- and T-cell epitopes of the Per a 6 and Bla g 6 allergens. Three immunoinformatics tools, the DNAStar Protean system, the Bioinformatics Predicted Antigenic Peptides system and the BepiPred 1.0 server, were used to predict the potential B-cell epitopes, while Net-MHCIIpan-2.0 and NetMHCII-2.2 were used to predict the T-cell epitopes of the two allergens. As a result, seven peptides were predicted in the Per a 6 allergen and seven peptides were predicted in the Bla g 6 allergen in the B-cell epitope predictions. In the T-cell prediction, the Per a 6 allergen was predicted to have nine strongly binding nonamer core epitope sequences (IC50