ONE-CARBON METABOLISM IN LECTIN-ACTIVATED HUMAN-LYMPHOCYTES
ONE-CARBON METABOLISM IN LECTIN-ACTIVATED HUMAN-LYMPHOCYTES
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DOI：
10.1016/0003-9861(85)90627-7
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发表时间：
1985-01-01
影响因子：
3.9
通讯作者：
MCCAIRNS, E
中科院分区：
文献类型：
--
作者：
ROWE, PB;SAUER, D;MCCAIRNS, E
丝氨酸是凝集素介导的人外周血淋巴细胞转化所必需的氨基酸，因为该细胞不能通过磷酸化途径或通过逆转丝氨酸羟甲基转移酶反应来合成足够数量的丝氨酸来满足代谢需求。细胞内丝氨酸水平受到严格控制，细胞转化的最佳培养基浓度落在一个相对较窄的范围内。丝氨酸的3-C原子是嘌呤和嘧啶核苷酸生物合成所需的1-C单位的主要来源，但丝氨酸的缺乏和高水平的中等丝氨酸似乎都会对蛋白质的合成产生关键影响。尽管另一种1-C单位的来源，如在培养基中高水平的甲酸盐，可以部分逆转丝氨酸剥夺的影响，但唯一的另一种可证明的1-C单位的来源，色氨酸，需要丝氨酸来转化，并参与少量磷脂酰胆碱的合成，尽管这种磷脂大部分是由补充血清的培养基中的胆碱和溶血磷脂酰胆碱提供的。
Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The level of intracellular serine is tightly regulated, and the culture medium concentration for optimum cellular transformation falls within a relatively narrow range. The 3-C atom of serine is the major source of 1-C units required for purine and pyrimidine nucleotide biosynthesis, but the key effect of both serine deprivation and of high medium serine levels would appear to be on protein synthesis. Although an alternative source of 1-C units, as provided by high levels for formate in the culture medium, can partially reverse the effects of serine deprivation, the only other demonstrable source of 1-C units, tryptophan, requires serine for its transformation and is involved in the synthesis of a small amount of phosphatidylcholine, although most of this phospholipid is provided by choline and lysophosphatidylcholine from the serum-supplemented culture medium.