Sepsis-induced lung inflammation is modulated by insulin.
Sepsis-induced lung inflammation is modulated by insulin.
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DOI：
10.1186/1471-2466-14-177
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发表时间：
2014-11-15
影响因子：
3.1
通讯作者：
Jancar S
中科院分区：
文献类型：
--
作者：
Filgueiras LR;Capelozzi VL;Martins JO;Jancar S
我们之前的研究表明，糖尿病大鼠更容易患脓毒症，但继发于脓毒症的急性肺损伤（ALI）不如非糖尿病大鼠严重。在本研究中，我们进一步研究了糖尿病大鼠脓毒症继发ali及胰岛素治疗的效果。四氧嘧啶诱导雄性Wistar大鼠糖尿病，盲肠结扎穿刺手术（CLP）诱导脓毒症。一些糖尿病大鼠给予中性鱼精蛋白Hagedorn （NPH）胰岛素（4 IU, s.c）。CLP前2小时。6 h后，检测肺水肿、细胞浸润及支气管肺泡灌洗液（BAL）中前列腺素e2 （PGE2）水平。结果证实，糖尿病脓毒症大鼠白细胞浸润和水肿较轻。胰岛素治疗后，糖尿病患者的肺部炎症增加到与非糖尿病患者相当的水平。脓毒症合并糖尿病患者血清PGE2的BAL浓度也较低，胰岛素治疗后升高。脓毒症后肺实质中早期成纤维细胞激活，通过转化生长因子(TGF)-β和平滑肌肌动蛋白（α-SMA）表达增加以及肌成纤维细胞形态细胞数量增加来评估。这些事件在糖尿病大鼠中显著降低，在胰岛素治疗后增加。结果表明，胰岛素调节糖尿病大鼠早期炎症和肌成纤维细胞分化。
We have previously shown that diabetic rats are more susceptible to sepsis, but that the Acute lung injury (ALI) secondary to sepsis is less intense than in non-diabetics. In the present study, we further investigated the ALI-secondary to sepsis in diabetic rats and the effect of insulin treatment. Diabetes was induced in male Wistar rats by alloxan and sepsis by cecal ligation and puncture surgery (CLP). Some diabetic rats were given neutral protamine Hagedorn (NPH) insulin (4 IU, s.c.) 2 h before CLP. Six h later, the lungs were examined for edema, cell infiltration and prostaglandin-E2 (PGE2) levels in the bronchoalveolar lavage (BAL). The results confirmed that leukocyte infiltration and edema were milder in diabetic rats with sepsis. After insulin treatment, the lung inflammation in diabetics increased to levels comparable to the non-diabetics. The BAL concentration of PGE2 was also lower in diabetics with sepsis, and increased after insulin treatment. Sepsis was followed by early fibroblast activation in the lung parenchyma, evaluated by increased transforming growth factor (TGF)-β and smooth muscle actin (α-SMA) expression, as well as an elevated number of cells with myofibroblasts morphology. These events were significantly lower in diabetic rats and increased after insulin treatment. The results show that insulin modulates the early phase of inflammation and myofibroblast differentiation in diabetic rats.
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DOI：
10.1186/cc7717
发表时间：
2009
期刊：
Critical care (London, England)
影响因子：
--
作者：
Esper AM;Moss M;Martin GS
通讯作者：
Martin GS
影响因子：
3.3
作者：
Menezes, SLS;Bozza, PT;Rocco, PRM
通讯作者：
Rocco, PRM
影响因子：
3.7
作者：
Filgueiras LR Jr;Martins JO;Serezani CH;Capelozzi VL;Montes MB;Jancar S
通讯作者：
Jancar S
DOI：
10.1152/ajplung.00298.2002
发表时间：
2003-09-01
影响因子：
4.9
作者：
Xu, YD;Hua, JS;Khalil, N
通讯作者：
Khalil, N
影响因子：
8.8
作者：
Yu, Shun;Christiani, David C.;Gong, Michelle Ng
通讯作者：
Gong, Michelle Ng