A simple and inexpensive high resolution color ratiometric planar optode imaging approach: application to oxygen and pH sensing
A simple and inexpensive high resolution color ratiometric planar optode imaging approach: application to oxygen and pH sensing
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DOI：
10.4319/lom.2011.9.348
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Glud, Ronnie N.
中科院分区：
文献类型：
--
作者：
Larsen, Morten;Borisov, Sergey M.;Glud, Ronnie N.
提出了一种简单、高分辨率的彩色平面光电成像方法。该方法简单、廉价、通用性强，可用于研究一系列分析物的二维分布和动力学。该成像方法利用标准商用数字单镜头反射相机的内置滤色器，仅使用一个激发光源即可同时记录不同颜色(红、绿、蓝)的发光体发射光。使用记录在单个图像中的不同颜色的强度之间的比率可以计算出分析物的浓度。通过使用易于合成的传感器获得海洋沉积物中O-2和pH分布的高分辨率数据，证明了该方法的稳健性。传感器依赖于铂(II)八乙基卟啉(PtOEP)和亲脂性8-羟基-1，3，6-吡喃三磺酸三钠(HPTS)盐衍生物分别用于O2和pH的测量。这两种指示剂的亮度通过利用供体分子(Macrolex黄香豆素)的能量转移而显著增强。此外，施主的辐射可作为氧气传感器的内部参考。这种方法依赖于半透明传感器，便于在测量过程中对传感器后面的沉积物进行目视检查。用于数据采集和校准的软件将从作者处获得，而所有硬件均可从一系列商业来源获得。整个测量系统的总成本约为3000美元。
A simple, high resolution colormetric planar optode imaging approach is presented. The approach is simple and inexpensive yet versatile, and can be used to study the two-dimensional distribution and dynamics of a range of analytes. The imaging approach utilizes the inbuilt color filter of standard commercial digital single lens reflex cameras to simultaneously record different colors (red, green, and blue) of luminophore emission light using only one excitation light source. Using the ratio between the intensity of the different colors recorded in a single image analyte concentrations can be calculated. The robustness of the approach is documented by obtaining high resolution data of O-2 and pH distributions in marine sediments using easy synthesizable sensors. The sensors rely on the platinum(II) octaethylporphyrin (PtOEP) and lipophilic 8-Hydroxy-1,3,6-pyrenetrisulfonic acid trisodium (HPTS) salt derivate for O-2 and pH measurements, respectively. The brightness of both indicators is dramatically enhanced by making use of energy transfer from a donor molecule (Macrolex yellow coumarin). Furthermore, the emission from the donor serves as an internal reference for the O-2 sensor. The approach relies on semitransparent sensors, facilitating visual inspection of the sediment behind the sensors during measurements. Software for data acquisition and calibration will be available from the authors, whereas all hardware is available from a range of commercial sources. The total cost of the complete measuring system is approximately $3000 US.