Suppressed cytokine production in whole blood cultures may be related to iron status and hepcidin and is partially corrected following weight reduction in morbidly obese pre-menopausal women.
Suppressed cytokine production in whole blood cultures may be related to iron status and hepcidin and is partially corrected following weight reduction in morbidly obese pre-menopausal women.
复制标题
全血培养物中细胞因子产生的抑制可能与铁状态和铁调素有关，并且在病态肥胖的绝经前女性体重减轻后得到部分纠正。
DOI：
10.1016/j.cyto.2010.11.008
复制
发表时间：
2011
期刊：
影响因子：
3.8
通讯作者：
Fantuzzi,Giamila
中科院分区：
文献类型：
--
作者：
Tussing-Humphreys,Lisa;Pini,Maria;Ponemone,Venkatesh;Braunschweig,Carol;Fantuzzi,Giamila
目的探讨肥胖与非肥胖妇女体外全血(WB)细胞因子的产生及其与铁状态和血清海普西丁的关系。17例肥胖者(体重指数：46.6±7.9 kg/m2)和19例非肥胖者(体重指数：22.5±3.0 kg/m2)，均为绝经前妇女；血红蛋白、年龄和种族的频率相匹配。测量在基线对照和体外刺激的基础上，从肝素化的白细胞培养物中提取IL-6、IL-10、IL-22、干扰素γ和肿瘤坏死因子α。结果与非肥胖组比较，肥胖组外周血刺激产生IL-6、肿瘤坏死因子α、干扰素γ降低，IL-22升高，IL-10无明显变化。此外，体外刺激IL-6和肿瘤坏死因子α的产生恢复正常，但干扰素γ的产生在限制性减肥手术后体重下降的情况下保持不变。肥胖妇女血清转铁蛋白受体(铁状态的标志)和血清海普西汀水平与体外刺激的干扰素γ的产生呈正相关。结论绝经前病态肥胖妇女外周血中脂多糖和滋阴颗粒刺激的细胞因子的产生发生了改变。显著的体重减轻导致了一些但不是所有观察到的改变的正常化。此外，铁状态和血清海普西丁与体外脂多糖和ZY刺激的干扰素γ在肥胖相关。
ObjectiveAssess ex vivo whole-blood (WB) cytokine production and its association with iron status and serum hepcidin in obese versus non-obese women. Determine the change in ex vivo WB cytokine production 6months after restrictive bariatric surgery in the obese group.SubjectsSeventeen obese (BMI: 46.6±7.9kg/m2) and 19 non-obese (BMI: 22.5±3.0kg/m2), pre-menopausal women; frequency matched for hemoglobin, age and race.MeasurementsAt baseline control and ex vivo stimulated IL-6, IL-10, IL-22, IFNγ, and TNFα from heparinized WB cultures, hemoglobin from finger-stick and transferrin receptor, hepcidin, CRP, IL-6, HOMA-IR from fasted serum samples and anthropometric parameters were assessed in the women. All parameters were reassessed 6-months following restrictive bariatric surgery in the obese women only.ResultsWhole blood ex vivo LPS and ZY stimulated production of IL-6, TNFα, and IFNγ was reduced, IL-22 increased, and IL-10 was unaffected in obese compared with the non-obese women. Furthermore, ex vivo stimulated production of IL-6 and TNFα normalized, but IFNγ production remained unchanged with weight loss following restrictive bariatric surgery. In the obese women, serum transferrin receptor (a marker of iron status) and serum hepcidin were correlated with ex vivo stimulated IFNγ production at baseline.ConclusionEx vivo LPS and ZY stimulated cytokine production from WB cultures was altered in pre-menopausal women with morbid obesity. Significant weight loss resulted in normalization of some but not all observed alterations. Furthermore, iron status and serum hepcidin were associated with ex vivo LPS and ZY stimulated IFNγ in obesity.