Functional Analysis of MCNA, a Gene Encoding a Catalytic Subunit of Calcineurin, in the Rice Blast Fungus Magnaporthe oryzae
Functional Analysis of MCNA, a Gene Encoding a Catalytic Subunit of Calcineurin, in the Rice Blast Fungus Magnaporthe oryzae
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DOI：
10.4014/jmb.0804.268
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Lee, Yong-Hwan
中科院分区：
文献类型：
--
作者：
Jinhee, Choi;Kim, Yangseon;Lee, Yong-Hwan
稻瘟病菌（Magnaporthe oryzae）是稻瘟病的病原菌，它会形成一种特殊的侵染结构，称为附着胞，这对于侵染和感染寄主植物至关重要。药理学数据表明，钙/钙调蛋白依赖性信号传导参与了这种真菌附着胞的形成。为了在分子水平上了解钙/钙调蛋白激活的蛋白磷酸酶在附着胞形成中的作用，对稻瘟病菌中编码钙调神经磷酸酶催化亚基的基因MCNA进行了功能鉴定。表达MCNA正义/反义RNA的转化体在菌丝生长、产孢、附着胞形成和致病性方面均显著降低。MCNA的cDNA能功能性互补酿酒酵母（Saccharomyces cerevisiae）的钙调神经磷酸酶缺失菌株（cmp1::LEU2 cmp2::HIS3）。这些数据表明，钙调神经磷酸酶A在稻瘟病菌与侵染相关的形态发生和致病性的信号传导途径中发挥着重要作用。
Magnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulin-dependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection-related morphogenesis pathogenicity of M. oryzae.