Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer
Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer
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DOI：
10.1158/0008-5472.can-05-4540
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发表时间：
2006-07-15
期刊：
影响因子：
11.2
通讯作者：
Takata, Takashi
中科院分区：
文献类型：
--
作者：
Kudo, Yasusei;Ogawa, Ikuko;Takata, Takashi
头颈部鳞状细胞癌（HNSCC）是人类最常见的癌症类型之一。通常，HNSCC细胞表现出持续的侵袭，经常导致局部复发和远处淋巴转移。然而，与HNSCC的侵袭和转移相关的分子机制仍然知之甚少。在这里，我们通过比较亲本HNSCC细胞和高侵袭性克隆之间的基因表达谱，确定骨膜蛋白为HNSCC中的侵袭促进因子。事实上，骨膜蛋白过表达促进HNSCC细胞的体外和体内侵袭和锚定非依赖性生长。此外，在原位HNSCC小鼠模型中，过表达骨膜蛋白的细胞通过其侵袭性自发转移到颈部淋巴结和肺。有趣的是，与正常组织相比，骨膜蛋白在HNSCC中高度表达，并且骨膜蛋白表达水平与HNSCC病例的侵袭性密切相关。总之，这些发现表明骨膜蛋白在HNSCC的侵袭和锚定非依赖性生长中起重要作用。
Head and neck squamous cell carcinoma (HNSCC) is one of the most common types of human cancer. Typically, HNSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. However, molecular mechanisms associated with the invasion and metastasis of HNSCC remain poorly understood. Here, we identified periostin as an invasion-promoting factor in HNSCC by comparing the gene expression profiles between parent HNSCC cells and a highly invasive clone. Indeed, periostin overexpression promoted invasion and anchorage-independent growth both in vitro and in vivo in HNSCC cells. Moreover, periostin-overexpressing cells spontaneously metastasized to cervical lymph nodes and to the lung through their aggressive invasiveness in an orthotopic mouse model of HNSCC. Interestingly, periostin was highly expressed in HNSCCs in comparison with normal tissues, and the level of periostin expression was well correlated with the invasiveness of HNSCC cases. In summary, these findings suggest that periostin plays an important role in the invasion and anchorage-independent growth of HNSCC.