Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4+8+ thymocytes in vitro.
Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4+8+ thymocytes in vitro.
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具有护理活性的胸腺基质细胞克隆支持小鼠 CD4 8 胸腺细胞的体外生长和分化。
DOI：
10.4049/jimmunol.145.12.4012
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发表时间：
1990
影响因子：
4.4
通讯作者：
S. Habu
中科院分区：
文献类型：
--
作者：
T. Nishimura;Y. Takeuchi;Y. Ichimura;X. Gao;A. Akatsuka;N. Tamaoki;H. Yagita;K. Okumura;S. Habu
胸腺基质细胞克隆 TNC-R3.1 细胞是从自发性 AKR/J 小鼠胸腺瘤中建立的。 TNC-R3.1细胞与胸腺护理细胞具有相似的特性，与正常胸腺细胞亚群形成独特的复合物。流式细胞术分析表明CD4+8+和CD4-8-未成熟胸腺细胞优先与TNC-R3.1基质细胞克隆相互作用。与 TNC-R3.1 基质细胞克隆相互作用的 CD4+8+ 胸腺细胞比不相互作用的 CD4+8+ 胸腺细胞含有更高比例的大尺寸和循环 T 细胞。正如普遍接受的那样，CD4+8+ 胸腺细胞对任何刺激（例如 IL-2、抗 CD3 mAb (2C11) 或 IL-2 加 2C11）均没有反应。然而，在次优剂量的 IL-2 存在下，在 TNC-R3.1 基质细胞单层上培养分离的 CD4+8+ 胸腺细胞会诱导显着的细胞生长。此外，在该共培养系统中添加2C11和IL-2导致胸腺细胞的增殖反应急剧增加。流式细胞术分析显示，TNC-R3.1上的增殖细胞来源于CD4+8+胸腺细胞，大部分为TCR-αβ+CD3+CD4-8+T细胞。这些结果提供了体外证据，表明CD4+8+胸腺细胞处于T细胞成熟的中间阶段，并且TNC-R3.1基质细胞克隆诱导CD4+8+胸腺细胞生长和分化为CD4-8+T细胞。
Thymic stromal cell clone, TNC-R3.1 cell, was established from spontaneous AKR/J mouse thymoma. TNC-R3.1 cell, which has the similar properties to thymic nurse cells, formed a unique complex with normal thymocyte subpopulations. Flow cytometry analysis demonstrated that CD4+8+ and CD4-8- immature thymocytes preferentially interacted with TNC-R3.1 stromal cell clone. CD4+8+ thymocytes, which interacted with TNC-R3.1 stromal cell clone, contained a higher proportion of large size and cycling T cells than did noninteracting CD4+8+ thymocytes. As is generally accepted, CD4+8+ thymocytes did not respond to any stimulation such as IL-2, anti-CD3 mAb (2C11), or IL-2 plus 2C11. However, culture of isolated CD4+8+ thymocytes on TNC-R3.1 stromal cell monolayer in the presence of suboptimal dose of IL-2 induced a significant cell growth. Moreover, the addition of 2C11 and IL-2 into this coculture system resulted in a dramatic increase of the proliferative response of thymocytes. Flow cytometry analysis showed the proliferating cells on TNC-R3.1, which originated from CD4+8+ thymocytes, were mostly TCR-alpha beta+ CD3+CD4-8+ T cells. These results provide in vitro evidence that CD4+8+ thymocytes are at an intermediate stage of T cell maturation and TNC-R3.1 stromal cell clone induces the growth and differentiation of CD4+8+ thymocytes into CD4-8+ T cells.