Mycosporine-like amino acids extracted from scallop (Patinopecten yessoensis) ovaries:: UV protection and growth stimulation activities on human cells
Mycosporine-like amino acids extracted from scallop (Patinopecten yessoensis) ovaries:: UV protection and growth stimulation activities on human cells
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DOI：
10.1007/s10126-007-9043-z
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Ishihara, Kenji
中科院分区：
文献类型：
--
作者：
Oyamada, Chiaki;Kaneniwa, Masaki;Ishihara, Kenji
虾夷扇贝（Patinopecten yessoensis）在日本被广泛养殖和登陆。在加工扇贝的过程中，大量的内脏和贝壳被作为工业废物排放。为了减少对环境的负担，需要废物的有效利用和处置方法。因此，我们从扇贝内脏器官中筛选出有用的物质，并从扇贝卵巢中发现了紫外线吸收物质。通过紫外吸收光谱、电喷雾质谱（ESI-MS）、电喷雾质谱/质谱（ESI-MS/MS）和核磁共振（NMR）等方法，鉴定了3种具有紫外吸收的类菌孢素氨基酸（mycosporine-like amino acids，MAAs）：shinorine、porphyra-334（P-334）和mycosporine-glycine。为了研究MAAs是否可以作为人体细胞的紫外线保护剂，我们研究了三种MAAs对人成纤维细胞免受紫外线照射的保护作用。所有三种检查的MAA保护细胞免受UV诱导的细胞死亡。特别是，霉菌素-甘氨酸具有最强的效果。此外，我们发现MAAs对人皮肤成纤维细胞的增殖具有促进作用。从这些结果中发现，从扇贝卵巢中分离的三种MAA对人体细胞具有抗紫外线的保护作用。MAAs作为紫外线防护剂和细胞增殖活化剂在化妆品和盥洗用品中具有潜在的应用前景。
Scallops (Patinopecten yessoensis) are extensively cultured and landed in Japan. During the processing of scallops, large amounts of internal organs and shells are discharged as industrial wastes. To reduce the burden on the environment, effective utilization and disposal methods of the wastes are required. Therefore, we have screened for useful materials in scallop internal organs, and found ultraviolet (UV) absorbing compounds from scallop ovaries. Based on UV absorption, electrospray ionization-mass spectrometry (ESI-MS), ESI-MS/MS, and nuclear magnetic resonance (NMR) spectra, three UV absorbing compounds were identified as mycosporine-like amino acids (MAAs): shinorine, porphyra-334 (P-334), and mycosporine-glycine. To investigate whether MAAs can act as a UV protector for human cells, we examined the protective effects of the three MAAs on human fibroblast cells from UV irradiation. All of the three examined MAAs protected the cells from UV-induced cell death. In particular, mycosporine-glycine had the strongest effect. Further, we found a promotion effect of MAAs on the proliferation of human skin fibroblast cells. From these results, it was found that the three MAAs isolated from scallop ovaries have a protective effect on human cells against UV light. MAAs have potential applications in cosmetics and toiletries as a UV protectors and activators of cell proliferation.