A novel electrochemiluminescence immunosensor based on functional beta-cyclodextrin-ferrocene host-guest complex with multiple signal amplification
A novel electrochemiluminescence immunosensor based on functional beta-cyclodextrin-ferrocene host-guest complex with multiple signal amplification
复制标题
一种基于具有多重信号放大功能的功能性β-环糊精-二茂铁主客体复合物的新型电化学发光免疫传感器
DOI：
10.1016/j.snb.2017.11.171
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yuan Ruo
中科院分区：
文献类型：
--
作者：
Xie Xiyue;Wang Haijun;Zhang Li;Liu Yuting;Chai Yaqin;Yuan Yali;Yuan Ruo
The shortened distance between luminophor and coreactant can effectively enhance luminous efficiency in electrochemiluminescence (ECL) system owing to the improvement of electron transfer rate. Here, a novel sandwich-type ECL immunosensor based on N-(aminobutyl)-N-(ethylisoluminol) (ABEI) as luminophor was constructed by integrating hollow gold nanoparticles (HAuNPs),l-cysteine (l-Cys) and ferrocene (Fc) in a complex for multiple signal amplification. The HAuNPs with large specific surface areas and superior conductivity were first used as scaffolds to load abundant coreactantl-Cys, aiming to further assemble catalyzer Fc stably. Through host-guest recognition, the β-cyclodextrin (β-CD) functionalized ABEI thus subsequently decorated on Fc, bringing in shortened distance between luminophor ABEI and coreactantl-Cys with high ECL signal. Moreover, the catalyzer Fc and HAuNPs could dramatically promote decomposition of another coreactant H2O2to produce abundant superoxide radical (O2radical dotˉ) and hydroxyl radical (OHradical dot) for further signal amplification. Taking β2-microglobulin (β2 M) as model analyte, the developed immunosensor exhibited a superior performance for detection β2 M in the range of 1.0 × 10−3–1.0 × 103ng mL−1with a detection limit of 2.6 × 10−1pg mL−1. The developed strategy realized solid-state ECL detection and multiple ECL signal amplification, providing a novel sensitive detection method for β2 M, which may show a great promise for the clinical application.
