Primary culture of differentiating ovarian androgen-producing cells in defined medium.
Primary culture of differentiating ovarian androgen-producing cells in defined medium.
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DOI：
10.1016/s0021-9258(18)34752-5
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发表时间：
1982-04
期刊：
影响因子：
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通讯作者：
D. Magoffin;G. Erickson
中科院分区：
文献类型：
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作者：
D. Magoffin;G. Erickson
取25~26日龄雌性大鼠的间质细胞，在无血清培养液中培养。在有促黄体生成素或人绒毛膜促性腺激素的情况下，类固醇的合成大约是在没有它们的情况下的200倍。在分泌的类固醇中，96%是雄激素，其中98%是雄酮。促黄体生成素或绒毛膜促性腺激素对雄酮分泌的刺激作用呈剂量依赖性。前列腺素E_2、霍乱毒素和8-溴环腺苷以剂量依赖的方式模拟黄体生成素对雄酮分泌的影响。在体内实验中，注射人绒毛膜促性腺激素引起的雄激素反应模式与体外发现的相似。
Interstitial cells from female rats aged 25-26 days were cultured in a serum-free medium. In the presence of LH or HCG, but not FSH, steroid synthesis was about 200 times greater than in their absence. Of the steroid secreted, 96% was androgen, and of this 98% was androsterone. The stimulation of androsterone secretion by LH or HCG was dose-dependent. The effect of LH on androsterone secretion was mimicked, in a dose-dependent manner, by PGE2, cholera toxin and 8-bromo cAMP. In in vivo experiments, administration of HCG induced a pattern of androgen response similar to than found in vitro.