MARKER RESCUE MAPPING OF VACCINIA VIRUS TEMPERATURE-SENSITIVE MUTANTS USING OVERLAPPING COSMID CLONES REPRESENTING THE ENTIRE VIRUS GENOME
MARKER RESCUE MAPPING OF VACCINIA VIRUS TEMPERATURE-SENSITIVE MUTANTS USING OVERLAPPING COSMID CLONES REPRESENTING THE ENTIRE VIRUS GENOME
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DOI：
10.1016/0042-6822(86)90261-8
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发表时间：
1986-04-15
期刊：
影响因子：
3.7
通讯作者：
CONDIT, RC
中科院分区：
文献类型：
--
作者：
THOMPSON, CL;CONDIT, RC
构建了一组11个野生型（wt）牛痘病毒DNA的重叠粘粒克隆。这些克隆几乎涵盖了整个牛痘病毒基因组。这些克隆被用于通过标记拯救来定位牛痘病毒的温度敏感（ts）突变体。从代表32个测试互补组中的29个的突变体获得图谱位置。
A set of 11 overlapping cosmid clones of wild type (wt) vaccinia virus DNA was constructed. The clones together span almost the entire vaccinia virus genome. The clones were used to map temperature-sensitive (ts) mutants of vaccinia virus by marker rescue. Map positions were obtained from mutants representing 29 of 32 complementation groups tested.