Multidimensional liquid chromatography: a breakthrough in the assessment of physiological vitamin K levels.
Multidimensional liquid chromatography: a breakthrough in the assessment of physiological vitamin K levels.
复制标题
多维液相色谱：生理维生素 K 水平评估的突破。
DOI：
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发表时间：
1982
影响因子：
6.5
通讯作者：
Herman L.C. Steyaert
中科院分区：
文献类型：
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作者：
M. Lefevere;A. P. D. Leenheer;A. Claeys;I. Claeys;Herman L.C. Steyaert
由于现有的化学检测缺乏敏感性和/或选择性，维生素K缺乏症一直是通过测量其对血液凝固的影响来间接诊断的。据我们所知，我们使用了我们最近开发的多维液相色谱法，对人体血液中生理维生素K水平进行了首次系统调查。它可以明确地证明反式叶绿醌（维生素K1），并将其定量到每毫升血清0.5纳克（ng/ml）的水平。在健康成人中，平均血清浓度为2.6 ng/ml，正常范围为0.9至7.8 ng/ml。这些值显然是以对数正态分布的。
Owing to the lack of sensitivity and/or selectivity of the existing chemical assays, vitamin K deficiency has always been diagnosed indirectly by measuring its effect on blood coagulation. We used our recently developed multidimensional liquid chromatographic assay for what is, to the best of our knowledge, the first systematic investigation of physiological vitamin K levels in human blood. It allowed the unequivocal demonstration of trans-phylloquinone (vitamin K1) and its quantification down to a level of 0.5 nanogram per milliliter of serum (ng/ml. In healthy adults, a mean serum concentration of 2.6 ng/ml was found, with a normal range of 0.9 to 7.8 ng/ml. These values apparently are distributed in a log-normal way.