Neutrophils influence the level of antigen presentation during the immune response to protein antigens in adjuvants.
Neutrophils influence the level of antigen presentation during the immune response to protein antigens in adjuvants.
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DOI：
10.4049/jimmunol.1001289
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发表时间：
2010-09-01
期刊：
影响因子：
--
通讯作者：
Unanue ER
中科院分区：
文献类型：
--
作者：
Yang CW;Strong BS;Miller MJ;Unanue ER
中性粒细胞在用完全或不完全弗氏佐剂或明矾中的抗原免疫后调节抗原呈递。中性粒细胞在CD 4 T细胞和B细胞对鸡蛋白色溶菌酶（HEL）、卵清蛋白（OVA）和溶血素O（LLO）三种蛋白抗原的反应中起重要的负性作用。在缺乏抗体的情况下（仅在最初的24小时内用抗体耗尽，或使用遗传性贫血小鼠），细胞反应增加了数倍。CD 8反应不受影响或略有下降。呈递细胞和嗜中性粒细胞之间的抗原竞争，以及对携带抗原的树突状细胞（DC）的反应的影响被记录。中性粒细胞迅速进入引流淋巴结，持续数小时，主要位于边缘窦和浅表皮质。在那里，他们与DC和巨噬细胞建立了短暂的接触。此外，嗜中性粒细胞印在随后的DC-T细胞相互作用的质量上，尽管没有与它们物理接触：通过活体显微镜，抗原特异性T细胞和DC的聚集在血小板减少症小鼠中得到改善。因此，嗜中性粒细胞是专性细胞，短暂进入免疫部位并设定抗原呈递水平：短暂的耗竭可能对疫苗接种产生相当积极的影响。
Neutrophils modulated antigen presentation following immunization with antigens in complete or incomplete Freund's adjuvant or alum. The neutrophils had an important negative role in the CD4 T cell and B cell responses to three protein antigens, hen-egg white lysozyme (HEL), ovalbumin (OVA) and listeriolysin O (LLO). In their absence (by depleting with antibodies for only the first twenty-four hours, or using genetically neutropenic mice), the cellular responses increased several fold. The CD8 response was not affected or slightly decreased. Competition for antigen between the presenting cells and the neutrophils, as well as an effect on the response to antigen-bearing dendritic cells (DC) was documented. Neutrophils entered the draining lymph nodes, rapidly and for a brief period of several hours, localizing mainly to the marginal sinus and superficial cortex. There they established brief contacts with DC and macrophages. Moreover, neutrophils imprinted on the quality of the subsequent DC-T cell interactions, despite no physical contacts with them: by intravital microscopy, the clustering of antigen-specific T cells and DC was improved in neutropenic mice. Thus, neutrophils are obligate cells that briefly enter sites of immunization and set the level of antigen presentation: a brief depletion may have a considerably positive impact on vaccination.
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感染期间淋巴结中中性粒细胞迁移的动态。
影响因子：
32.4
作者：
Chtanova, Tatyana;Schaeffer, Marie;Han, Seong-Ji;van Dooren, Giel G.;Nollmann, Marcelo;Herzmark, Paul;Chan, Shiao Wei;Satija, Harshita;Camfield, Kristin;Aaron, Holly;Striepen, Boris;Robey, Ellen A.
通讯作者：
Robey, Ellen A.
影响因子：
15.9
作者：
Fadok, VA;Bratton, DL;Henson, PM
通讯作者：
Henson, PM
影响因子：
4.4
作者：
Bennouna, S;Bliss, SK;Denkers, EY
通讯作者：
Denkers, EY
影响因子：
4.4
作者：
Martino, Angelo;Badell, Edgar;Winter, Nathalie
通讯作者：
Winter, Nathalie
DOI：
10.1073/pnas.91.10.4480
发表时间：
1994-05-10
影响因子：
11.1
作者：
DONG, F;HOEFSLOOT, LH;LOWENBERG, B
通讯作者：
LOWENBERG, B