Vasorelaxant and hypotensive effects of the extract and the isolated flavonoid rutin obtained from Polygala paniculata L.
Vasorelaxant and hypotensive effects of the extract and the isolated flavonoid rutin obtained from Polygala paniculata L.
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DOI：
10.1111/j.2042-7158.2010.01240.x
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Soares Santos, Adair Roberto
中科院分区：
文献类型：
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作者：
Lapa, Fernanda da Rocha;Soares, Kelly Cristina;Soares Santos, Adair Roberto
本研究旨在探讨远志水醇粗提物（HEPP）对大鼠离体和在体心血管系统的作用。方法在正常血压麻醉大鼠和主动脉环上进行该程序。关键发现当在内皮完整的主动脉环上进行测试时，HEPP（30-1000 μ g/ml）产生显著的非浓度依赖性舒张作用（类似于40%），通过与L-NAME孵育完全阻止（一氧化氮合酶抑制剂）（可溶性鸟苷酸环化酶抑制剂）和部分抑制四乙铵（TEA;一种非选择性钾通道阻滞剂）和Charybdotoxin（一种大电导和中电导钙激活钾通道阻滞剂）。相反，阿托品（一种毒蕈碱受体拮抗剂）或吡拉明（一种组胺H-1受体拮抗剂）则无作用。此外，在麻醉大鼠中口服HEPP（30-300 mg/kg）引起剂量依赖性和持续的肿胀作用。阿托品或TEA对这种作用没有影响，但在连续输注L-NAME或亚甲蓝的大鼠中强烈降低。此外，芦丁（1-3毫克/公斤），通过静脉途径也引起了剂量依赖性的肿胀作用，在rats.ConclusionsOur结果表明，从P.圆锥花序提取物诱导有效的肿胀和血管舒张作用，依赖于一氧化氮/鸟苷酸环化酶途径。这些影响可能有关，至少部分，在这个提取物中的芦丁含量。
ObjectivesThis study aimed to investigate the in-vitro and in-vivo cardiovascular effects of the crude hydroalcoholic extract from Polygala paniculata (HEPP) in rats.MethodsThe procedures were performed on aortic rings and on normotensive anaesthetized rats.Key findingsWhen tested in endothelium-intact aorta rings, HEPP (30-1000 mu g/ml) produced a significant non-concentration-dependent relaxing effect (similar to 40%), which was completely prevented by incubation with L-NAME (nitric oxide synthase inhibitor), ODQ (soluble guanylate cyclase inhibitor) and partially inhibited by tetraethylammonium (TEA; a non-selective potassium channel blocker) and charybdotoxin (a large- and intermediate-conductance calcium-activated potassium channel blocker). In contrast, atropine (a muscarinic receptor antagonist) or pyrilamine(a histamine H-1 receptor antagonist) had no effect. Furthermore, oral administration of HEPP (30-300 mg/kg) in anaesthetized rats caused a dose-dependent and sustained hypotensive action. This effect was unchanged by atropine or TEA, but was strongly reduced in rats continuously infused with L-NAME or methylene blue. Moreover, rutin (1-3 mg/kg) administered by an intravenous route also caused a dose-dependent hypotensive effect in rats.ConclusionsOur results demonstrated that the extract obtained from P. paniculata induces potent hypotensive and vasorelaxant effects that are dependent on the nitric oxide/guanylate cyclase pathway. These effects could be related, at least in part, to the rutin contents in this extract.