Evidence for a shift in the Th-1 to Th-2 cytokine response associated with chronic stress and aging
Evidence for a shift in the Th-1 to Th-2 cytokine response associated with chronic stress and aging
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DOI：
10.1093/gerona/56.8.m477
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发表时间：
2001-08-01
影响因子：
5.1
通讯作者：
Kiecolt-Glaser, JK
中科院分区：
文献类型：
--
作者：
Glaser, R;MacCallum, RC;Kiecolt-Glaser, JK
背景许多研究表明，照顾痴呆症患者的慢性压力可能具有显着的免疫后果，如细胞免疫应答的下调/失调所示。利用流式细胞术测量产生Th-1、Tc 1和Th-2以及Tc 2细胞的细胞因子的CD-4（+）和CD-8（+）T淋巴细胞的百分比和绝对数量，我们比较了配偶照顾者和对照受试者。检测CD-4（+）和CD-8（+）淋巴细胞胞浆中IL-2、IFN-γ和IL-10的表达。应激和年龄与IFN-γ（+）/CD-8（+）、IL-2（+）/CD-8（+）细胞或IFN-γ（+）、IL-2（+）、CD-4+细胞的百分比或数量均无显著相关。然而，从照顾者获得的淋巴细胞中IL-10（+）细胞的百分比高于对照组。此外，应激与衰老对IL-10（+）/CD-4（+）和IL-10（+）/CD-8（+）细胞的显著交互作用表明，照料者与对照受试者之间的差异具有年龄依赖性;照料者与对照受试者之间的差异在年轻个体中显著大于老年个体。这些数据是一致的，与以前的报告，急性应激，并建议也可能有一个转变，从Th-1到Th-2反应与慢性应激源，如抑郁症。这种转变可能对个体对病原体的反应产生影响。
Background. A number of studies have shown that the chronic stress of caring for persons with dementia can have significant immunological consequences as demonstrated by the down-regulation/dysregulation of the cellular immune response.Methods. Utilizing flow cytometry to measure the percentages and absolute numbers of CD-4(+) and CD-8(+) T lymphocytes producing the cytokines indicative of Th-1, Tc1 and Th-2, and Tc2 cells, we compared spousal caregivers and control subjects. The expression of interleukin-2 (IL-2), interferon gamma (IFN-gamma), and interleukin-10 (IL-10) in the cytoplasm of CD-4(+) and CD-8(+) lymphocytes was assessed.Results. Neither stress nor age was significantly related to the percentage or number of IFN-gamma (+)/CD-8(+), IL-2(+)/CD-8(+) cells, or IFN gamma (+), IL-2(+), CD-4+ cells. However, the percentage of IL-10(+) cells was higher in lymphocytes obtained from caregivers than control subjects. In addition, the significant interaction between stress and aging for IL-10(+)/CD-4(+) and IL-10(+)/CD-8(+) cells demonstrated that the difference between caregivers and control subjects was age dependent; the difference between caregivers and control subjects was substantially larger in younger individuals than in older individuals.Conclusions. The data are consistent with previous reports on acute stress and suggest that there may also be a shift from a Th-1 to a Th-2 response associated with a chronic stressor such as caregiving. This shift could have implications for an individual's responses to pathogens.