Model for Microneurovascular Muscle Transplantation in the Dog
Model for Microneurovascular Muscle Transplantation in the Dog
复制标题
狗微神经血管肌肉移植模型
DOI：
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发表时间：
1986
影响因子：
3.6
通讯作者：
V. Edgerton
中科院分区：
文献类型：
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作者：
S. Das;S. Spector;Timothy A. Miller;T. Martin;V. Edgerton
先前的研究表明，成功移植骨骼肌以取代先前丧失的功能取决于移植组织的质量。在本实验中，仔细的显微神经血管外科技术大大提高了大尺寸肌肉移植成功的机会的可能性进行了测试，使用狗股薄肌平均75克的重量。股薄肌完全切除同侧和植入到其原来的位置（原位）通过重新连接肌腱的插入和起源。此外，神经残端的神经缝合术与血管蒂的修复一起使用显微外科技术进行沿着。大约1年后，原位移植物的重量约为对侧假手术股薄肌的70%。虽然移植肌肉的平均张力输出下降到控制值的60%左右，但三个最成功的移植肌肉产生了73%到88%的控制力。慢收缩氧化纤维数量的显著增加与移植肌肉最大缩短速度的轻微但显著的降低相关。当重复刺激时，移植物抵抗疲劳的能力与对照肌肉的耐力相似。这些结果表明，显微神经血管手术可能会促进移植的骨骼肌功能的更完整的恢复相对较大的尺寸。
Previous studies have suggested that successful transplantation of skeletal muscle to replace previously lost function depends on the mass of the transplanted tissue. In the present experiment, the possibility that careful microneurovascular surgical technique substantially improves the chances of successful transplantation of large-sized muscle was tested using dog gracilis muscle averaging 75 gm in weight. Gracilis muscles were completely excised ipsilaterally and were implanted into their original location (orthotopic) by reattaching tendons of insertion and origin. In addition, neurorrhaphies of nerve stumps were performed along with repair of the vascular pedicle using microsurgery techniques. After approximately 1 year, orthotopic transplants weighed about 70 percent of contralateral sham-operated gracilis muscles. Although average tension output of transplants declined to about 60 percent of control values, three of the most successfully transplanted muscles produced between 73 and 88 percent of control force. A significant increase in the number of slow-twitch-oxidative fibers was correlated with a slight but significant reduction in the maximal velocity of shortening of transplanted muscles. The ability of transplants to resist fatigue when repetitively stimulated was similar to the endurance capacity of control muscles. These results suggest that microneurovascular surgery may enhance the more complete restoration of function of transplanted skeletal muscles of relatively large size.