Macrophage scavenger receptor MARCO: In vitro and in vivo regulation and involvement in the anti-bacterial host defense
Macrophage scavenger receptor MARCO: In vitro and in vivo regulation and involvement in the anti-bacterial host defense
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DOI：
10.1016/s0165-2478(97)00077-1
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发表时间：
1997-06-01
影响因子：
4.4
通讯作者：
Kraal, G
中科院分区：
文献类型：
--
作者：
vanderLaan, LJW;Kangas, M;Kraal, G
最近，A 类清道夫受体家族的一个新的鼠类成员被克隆，命名为 MARCO [4]。清道夫受体具有广泛的配体特异性，能够结合各种物质，包括细菌细胞表面成分。受体 MARCO 由脾脏和淋巴中巨噬细胞的独特亚群表达。使用一组专门针对 MARCO 的单克隆抗体 (mAb)，我们研究了该受体的体外和体内调节。用细菌脂多糖 (LPS) 体外刺激小鼠巨噬细胞系 J774.2 可诱导 MARCO 表面表达上调。根据这一观察，在因细菌感染引起内毒素休克的小鼠中，肝脏、肺和脾中的巨噬细胞诱导MARCO表达，而这些细胞通常不表达该受体。我们发现两种抗 MARCO 单克隆抗体 ED29 和 ED31 能够阻断配体结合。他们抑制了表达 MARCO 的 CHO 细胞对热灭活大肠杆菌的摄取。需要进一步的实验来证实 MARCO 在抗菌宿主防御中的重要性。 (C) 1997 Elsevier Science B.V.
Recently, a novel murine member of the scavenger receptor class A family, designated MARCO, has been cloned [4]. Scavenger receptors have a characteristic broad ligand specificity and are able to bind various substances, including bacteria cell surface components. The receptor MARCO is expressed by a distinct subset of macrophages in spleen and lymph codes. Using a panel of monoclonal antibodies (mAbs) specifically directed against MARCO, we investigated the regulation of this receptor in vitro and in vivo. Stimulation of the mouse macrophage cell line J774.2 in vitro with bacterial lipopolysaccharides (LPS) induces upregulation of MARCO surface expression. In accordance with this observation, in mice suffering from endotoxin shock caused by bacterial infection, expression of MARCO is induced on macrophages in the liver, lung and spleen, which normally do not express this receptor. We found that two of the anti-MARCO mAbs, ED29 and ED31, were able to block ligand binding. They inhibited the uptake of heat-killed Escherichia coli by MARCO expressing CHO cells. Further experiments will be needed to confirm the importance of MARCO in the anti-bacterial host defense. (C) 1997 Elsevier Science B.V.