Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice.
Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice.
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DOI：
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发表时间：
2009-06
影响因子：
2
通讯作者：
Chisa Nagakura;K. Hayashi;Ming Zhao;Kensuke Yamauchi;N. Yamamoto;H. Tsuchiya;K. Tomita;M. Bouvet;R. Hoffman
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文献类型：
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作者：
Chisa Nagakura;K. Hayashi;Ming Zhao;Kensuke Yamauchi;N. Yamamoto;H. Tsuchiya;K. Tomita;M. Bouvet;R. Hoffman
我们在这里报告了一种肿瘤靶向治疗胰腺癌的策略，该策略使用了一种改良的营养缺陷型沙门氏菌。转基因鼠伤寒沙门氏菌需要精氨酸和亮氨酸两种氨基酸。这些突变阻止了正常组织的生长，但不会降低肿瘤细胞中细菌的毒力。将表达绿色荧光蛋白(GFP)的肿瘤靶向性鼠伤寒沙门氏菌A1-R株接种于裸鼠原位表达红色荧光蛋白(RFP)的人胰腺癌细胞。经过7天的治疗，胰腺癌在没有化疗或任何其他治疗的情况下已经消退。这一新策略展示了细菌靶向治疗胰腺癌的临床潜力。
We report here a tumor-targeting strategy for pancreatic cancer using a modified auxotrophic strain of Salmonella typhimurium. The genetically-modified strain of S. typhimurium requires the amino acids arginine and leucine. These mutations preclude growth in normal tissue but do not reduce bacterial virulence in tumor cells. The tumor-targeting strain of S. typhimurium, termed A1-R and expressing green fluorescent protein (GFP), was administered to an orthotopic human pancreatic tumor expressing red fluorescent protein (RFP) in nude mice. After 7 days of treatment, the pancreatic cancer had regressed without the need of chemotherapy or any other treatment. This new strategy demonstrates the clinical potential of bacterial targeting for pancreatic cancer.