Postprandial Monocyte Activation in Individuals With Metabolic Syndrome
Postprandial Monocyte Activation in Individuals With Metabolic Syndrome
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DOI：
10.1210/jc.2016-2732
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发表时间：
2016-11-01
影响因子：
5.8
通讯作者：
Ballantyne, Christie M.
中科院分区：
文献类型：
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作者：
Khan, Ilvira M.;Pokharel, Yashashwi;Ballantyne, Christie M.
背景：餐后高脂血症被认为通过诱导单核细胞的促炎性改变而促进动脉粥样硬化的形成。代谢综合征(MS)患者具有较高的血甘油三酯浓度和甘油三酯清除延迟，因此可能增加发生动脉粥样硬化的风险。目的：我们的目的是检测肥胖和MS患者和健康对照组的空腹水平和高脂餐对单核细胞亚群表型的影响。设计、地点、参与者、干预：纳入肥胖和多发性硬化症患者以及性别和年龄匹配的健康对照组。参与者在隔夜禁食(基线)后以及在摄入高脂肪食物后3小时和5小时采集血液。主要观察指标：基础状态下3种单核细胞亚群的活化标志物水平和餐后炎症反应。结果：基础状态下肥胖和MS患者外周血中含脂泡沫单核细胞比例高于对照组，且与空腹甘油三酯水平呈正相关。此外，MS组非经典单核细胞计数增加，CD11c、CX3CR1和人类白细胞抗原-DR水平升高，循环中经典单核细胞CCR5和肿瘤坏死因子-α水平升高。两组餐后甘油三酯的升高与富含脂质的泡沫单核细胞的上调相平行。MS患者非经典单核细胞中IL-1β+和肿瘤坏死因子-α+细胞的CD11百分率在餐后显著升高。结论：与对照组相比，肥胖和MS患者的空腹和餐后单核细胞脂质蓄积和活化增加。
Context: Postprandial hyperlipidemia has been suggested to contribute to atherogenesis by inducing proinflammatory changes in monocytes. Individuals with metabolic syndrome (MS), shown to have higher blood triglyceride concentration and delayed triglyceride clearance, may thus have increased risk for development of atherosclerosis.Objective: Our objective was to examine fasting levels and effects of a high-fat mealonphenotypes of monocyte subsets in individuals with obesity and MS and in healthy controls. Design, Setting, Participants,Intervention: Individuals with obesity and MS and gender-and age-matched healthy controls were recruited. Blood was collected from participants after an overnight fast (baseline) and at 3 and 5 hours after ingestion of a high-fat meal. At each time point, monocyte phenotypes were examined by multiparameter flow cytometry.Main Outcome Measures: Baseline levels of activation markers and postprandial inflammatory response in each of the three monocyte subsets were measured.Results: At baseline, individuals with obesity and MS had higher proportions of circulating lipidladen foamy monocytes than controls, which were positively correlated with fasting triglyceride levels. Additionally, the MS group had increased counts of nonclassical monocytes, higher CD11c, CX3CR1, andhumanleukocyte antigen-DR levelsonintermediate monocytes, and higher CCR5 and tumor necrosis factor-alpha levels on classical monocytes in the circulation. Postprandial triglyceride increases in both groups were paralleled by upregulation of lipid-laden foamy monocytes. MS, but not control, subjectshadsignificant postprandial increases of CD11 candpercentages of IL-1 beta+ and tumor necrosis factor-alpha+ cells in nonclassical monocytes.Conclusions: Compared to controls, individuals with obesity and MS had increased fasting and postprandial monocyte lipid accumulation and activation.